Objective: To explore the significance of serum cytokeratin 18-M30 fragment (CK18-M30) and cytokeratin 18-M65 fragment (CK18-M65) levels in screening of fatty liver in physical examination population, and analysis of risk factors for fatty liver. Method: A total of 147 physical examiners from July 2020 to June 2022 in Taicang First People's Hospital were selected as the subjects of this study. The detection of fatty liver in physical examiners was recorded, and they were divided into fatty liver group (n=38) and non fatty liver group (n=109) according to whether they had fatty liver. The expression levels of CK18-M30 and CK18-M65 in serum of the two groups were compared, the factors influencing fatty liver were analyzed by univariate and multivariate logistic regression, and the application value of their expression levels in the diagnosis of fatty liver was analyzed with receiver operating characteristic (ROC). Result: Among 147 examiners, a total of 38 patients with fatty liver were detected, with a detection rate of 25.85% (38/147). The expression levels of CK18-M30 and CK18-M65 in the serum of the fatty liver group were higher than those of the non fatty liver group, the differences were statistically significant (P<0.05). The results of univariate analysis showed that there were no significant differences in gender, age, and the levels of tumor necrosis factor-α (TNF-α), C-reactive protein (CRP) and aspartate aminotransferase (AST) between the two groups (P>0.05). The body mass index (BMI), waist circumference, the proportion of diabetes, hypertension, hyperlipidemia and alanine aminotransferase (ALT) levels in the fatty liver group were significantly higher than those in the non fatty liver group, the differences were statistically significant (P<0.05). Multivariate logistic regression analysis showed that high BMI (OR=1.874), high waist circumference (OR=2.114), combined diabetes (OR=2.257), high CK18-M30 (OR=2.577), and high CK18-M65 (OR=1.974) were independent risk factors for fatty liver (P<0.05). The results of ROC analysis showed that the area under the curve (AUC) of CK18-M30 and CK18-M65 in the diagnosis of fatty liver was 0.885 (95%CI: 0.822, 0.948), significantly higher than 0.735 (95%CI: 0.636, 0.834) of CK18-M30 alone and 0.778 (95%CI: 0.704, 0.853) of CK18-M65 alone. Conclusion: High BMI, High waist circumference, combined diabetes, high CK18-M30 and high CK18-M65 are independent risk factors for fatty liver. The levels of CK18-M30 and CK18-M65 show abnormally high expression in the serum of patients with fatty liver, which has good clinical diagnostic effect in fatty liver, and can be used as effective indicators for screening fatty liver in the physical examination population.

Objective: To analyze the influence of hospital to family seamless model nursing on the stigma and negative psychology of patients undergoing enterostomy. Method: A total of 80 patients undergoing enterostomy admitted to the Department of General Surgery of the First Affiliated Hospital of Xiamen University from January 2021 to June 2022 were selected as the study objects. They were divided into control group and observation group according to random number table method, with 40 cases in each group. The control group was given routine nursing, and the observation group was given hospital to family seamless model nursing on the basis of control group. Stigma, negative psychology before and after nursing and gastrointestinal function recovery time were compared between the two groups. Result: After nursing, the scores of social exclusion, intrinsic shame and social isolation in the observation group were lower than those in the control group, and the differences were statistically significant (P<0.05). After nursing, depression score and anxiety score in the observation group were lower than those in the control group, and the differences were statistically significant (P<0.05). The first feeding time, first ostomy defecation time and first ostomy exhaust time in the observation group were significantly earlier than those in the control group, and the differences were statistically significant (P<0.05). Conclusion: The hospital to family seamless model nursing can effectively reduce the sense of shame and negative psychological emotions of patients, and is more conducive to promoting the recovery of gastrointestinal function of patients.

Migraine is a common chronic neurovascular disease in clinical practice, with repeated attacks, stubborn and difficult to heal as the main characteristics, to the patient's physical and psychological caused significant distress, greatly affecting the quality of life of patients. In recent years, the in-depth study of intestinal microecology theory has found that intestinal flora can participate in the occurrence and development of migraine through various ways. Based on the theory of "microbial-gut-brain axis", this paper discusses the four aspects of meridians, Qi and blood, emotions, phlegm turbidity, so as to provide the basis for the treatment of migraine by spleen and stomach in traditional Chinese medicine.

　　Constipation is a common clinical symptom, which often leads to anorectal diseases, colorectal cancer, acute myocardial infarction, cerebrovascular accidents and other diseases. It affects the daily life of patients and causes economic and social burden. The unique "holistic concept" and "syndrome differentiation and treatment" theoretical system of traditional Chinese medicine has advantages in the treatment of constipation. In clinical practice, chronic constipation is common in the form of asthenia constipation, mainly in the form of Qi deficiency and Yin deficiency, and mostly mixed with each other. Therefore, this paper briefly describes the methods of treating constipation with deficiency of both Qi and Yin in recent years, with a view to providing inspiration or improving the deficiency of the treatment of constipation with deficiency of both Qi and Yin.

Objective: To explore the effect of graded pain management based on numeric rating scales (NRS) in patients with subarachnoid hemorrhage (SAH) after surgery. Method: A total of 83 patients with SAH who underwent interventional embolization in Zhangzhou Hospital from January 2021 to January 2022 were enrolled in the study, the patients were divided into the control group (n=41) and the observation group (n=42) according to the random number table method. The control group was given routine pain management after SAH, and the observation group was given graded pain management based on NRS score. The pain score and self-efficacy score at different times, complications and satisfaction were compared between two groups. Result: There were no significant differences in NRS scores between two groups before and 10 d after intervention (P>0.05); the NRS scores of both groups decreased at 1 d, 3 d, 5 d and 10 d after intervention, the NRS scores in the two group at 1 d, 3 d and 5 d after intervention were significantly lower than those in the control group, the differences were statistically significant (P<0.05). There was no significant difference in the score of general self-efficacy scale (GSES) between two groups before intervention (P>0.05); the GSES scores in the observation group at 1 d, 3 d, 5 d and 10 d

Objective: To compare the differences in clinical pharmacodynamic characteristics between Ciprofol and Propofol. Method: From July to October 2021, 80 patients underwent internal fixation of distal upper limb fractures in the Affiliated Hospital of Panzhihua University were randomly divided into Ciprofol group and Propofol group, 40 cases in each group. After the completion of ultrasound-guided brachial plexus block, two groups were intravenously pumped with 2 mg/kg Propofol and 0.4 mg/kg Ciprofol for 1 min. The mean arterial pressure (MAP), heart rate (HR), blood oxygen saturation (SpO₂) and bispectral index (BIS) were recorded and compared between two groups before administration (T0) and 1 min (T1), 2 min (T2), 3 min (T3), 4 min (T4), 5 min (T5) after the start of pumping drugs; the induction time, consciousness recovery time and orientation recovery time of two groups were observed; in addition, the injection pain score and adverse reactions were compared between the two groups. Result: There were significant differences in MAP, HR, SpO₂ and BIS at different time points between two groups (P<0.05); MAP and SpO₂ at T1, T2, T3, T4, T5 in Propofol group were lower than those at T0, the differences were statistically significant (P<0.05); MAP at T1 and T2 in Ciprofol group were lower than those at T0, and SpO₂ at T1, T2, T3, T4 were lower than those at T0, the differences were statistically significant (P<0.05); MAP and SpO₂ at T1, T2, T3, T4, T5 of Ciprofol group were higher than those of Propofol group, the differences were statistically significant (P<0.05); HR at T1 and T2 of two groups were higher than those at T0, the differences were statistically significant (P<0.05); HR at T1 and T2 of Ciprofol group were lower than those of Propofol group, the differences were statistically significant (P<0.05); BIS at T1, T2, T3, T4 and T5 of two groups were lower than those at T0, the differences were statistically significant (P<0.05). The induction time, consciousness recovery time and orientation recovery time of Ciprofol group were significantly shorter than those of Propofol group, and the differences were statistically significant (P<0.05 ). The visual analogue scale (VAS) score and incidence of hypotension and hypoxemia in Ciprofol group were lower than those in Propofol group, and the differences were statistically significant (P<0.05). Conclusion: The anesthetic efficacy of 0.4mg/kg Ciprofol was equivalent to

Objective: Preclinical models often fail to capture the different heterogeneity of human malignant tumors, thus lacking clinical predictive ability. Patient derived tumor xenotransplantation (PDX) has become a powerful technique that preserves the molecular heterogeneity of its original samples. Among them, renal subcapsular xenotransplantation technology is a method of establishing a human cancer model in animals, which has received increasing attention in recent years. Based on this technology, medical researchers can study the biological characteristics, histological characteristics, and treatment responses of cancer by transplanting primary tumors obtained from patients into the renal capsule of mice, and then observing long-term reproduction and observation in mice. The article reviewed the progress and application of this technology in clinical and basic medical research in recent years, showcasing its advantages in the field of tumor treatment and research, providing reference for clinical and basic researchers in tumor prevention and treatment research.

Psoriasis is an inflammatory skin disease that occurs under the joint action of multiple factors such as genetics, environment, immunity etc. The interleukin-23 (IL-23) / T helper cell 17 (Th17) pathway is an important research achievement of the pathogenesis of psoriasis in clinical practice. Phosphodiesterase 4 (PDE4) inhibitors had a good blocking effect on cyclic adenosine monophosphate (cAMP) hydrolysis process, and effectively affected IL-23 secretion. This article briefly summarizes the pathogenesis of psoriasis, the mechanism of PDE4 inhibitors in the treatment of psoriasis and the progress of related PDE4 inhibitors drugs in the treatment of psoriasis.

Objective: To investigate the expression and significance of mutant P53 protein in the tissues of prophylactic salpingectomy. Method: A total of 929 patients who underwent laparoscopic total or subtotal hysterectomy and bilateral salpingectomy for benign uterine lesions in Xiamen Maternal and Child Health Care Hospital from May 2014 to September 2018 were collected, there were 827 cases of laparoscopic total hysterectomy+bilateral salpingectomy and 102 cases of laparoscopic subtotal hysterectomy+bilateral salpingectomy. The expression of mutant P53 protein in fallopian tube tissue was detected by immunohistochemistry. The factors affecting the expression of mutant P53 protein was analyzed, and followed up the occurrence of serous ovarian cancer after surgery. Result: There were no statistically significant differences in the expression of mutant P53 protein in different fallopian tube tissues among patients with benign uterine disease type, tubal ligation history, cesarean section history and age (P>0.05); however, there was significant difference in the expression of mutant P53 protein in different fallopian tube tissues among patients with the levels of carbohydrate antigen 125 (CA125) (P<0.05). logistic regression analysis showed that CA125 positive was a risk factor for mutant P53 positive protein expression (P<0.05). There was no serous ovarian cancer in the mutant P53 protein positive group after total uterus + bilateral salpingectomy and subtotal uterus + bilateral salpingectomy. Conclusion: The positive rate of mutant P53 protein expression in the tissues of prophylactic salpingectomy was 14.32%, and CA125 positive was a risk factor for mutant P53 positive protein expression, no serous ovarian cancer occurred in these patients after postoperative follow-up.

Objective: To explore the effect of Manchester pain management model (MPMM) combined with enabling education intervention on cesarean section parturient. Method: A total of 100 cases of cesarean section delivered in Women and Children's Hospital Affiliated to Xiamen University from October 2022 to October 2023 were selected, and divided into control group and study group according to different intervention methods, with 50 cases in each group. The control group was treated with routine intervention, and the study group was treated with MPMM combined with enabling education intervention. The degree of postoperative pain, postoperative recovery, negative emotion and exclusive breast-feeding were compared between the two groups. Result: At 8 h, 12 h and 24 h after operation, the numerical rating scale (NRS) scores at rest and exercise of the study group were lower than those of the control group, and the differences were statistically significant (P<0.05). The time of getting out of bed in the study group was earlier than that in the control group, and the difference was statistically significant (P<0.05). After the intervention, the Hamilton anxiety scale (HAMA) scores and Hamilton depression scale (HAMD) scores in the study group were lower than those in the control group, and the differences were statistically significant (P<0.05). The lactation initiation time of the study group was shorter than that of the control group, and the exclusive breast-feeding rate and breast-feeding self-efficacy scale short form (BSES-SF) score of the study group were higher than those of the control group, the differences were statistically significant (P<0.05). Conclusion: MPMM combined with enabling education intervention can reduce pain after uterine labor, promote postoperative recovery, improve maternal negative emotions, and increase the rate of exclusive breast-feeding.

Objective: To explore the efficacy of mindfulness therapy in schizophrenia rehabilitation period patients. Method: A total of 100 schizophrenic rehabilitation period patients admitted to Qinzhou Psychiatric Hospital from January to October 2022 were selected as the research objects. They were randomly divided into observation group and control group, with 50 cases in each group. Both groups were treated with the same antipsychotic drugs. The control group was administered with routine health education, and the observation group was administered with mindfulness therapy. The medication adherence rate, and anxiety scores, depression scores, quality of life scores before and after the intervention were compared between the two groups. Result: The medication adherence rate of the observation group was 98.00%, which was higher than 80.00% of the control group, the difference was statistically significant (P<0.05). After intervention, the self rating anxiety scale (SAS) score and self rating depression scale (SDS) score of the observation group were (46.63±5.44) points and (47.69±5.56) points respectively, which were lower than (51.61±6.30) points and (53.17±5.72) points of the control group, the differences were statistically significant (P<0.05). The total score of the schizophrenia quality of life scale (SQLS) in the observation group was (20.71±5.21) points, which was significantly lower than (23.86±5.65) points of the control group, and the difference was statistically significant (P<0.05). Conclusion: Implementing mindfulness therapy in schizophrenic rehabilitation period patients can effectively improve the medication adherence, alleviate the anxiety and depression symptoms, improve the cognitive abilities such as attention and memory, enhance the emotional regulation ability, and improve the quality of life after prognosis.

In recent years, chromosome karyotype analysis combined with copy number variation sequencing (CNV-seq) technology is a common means of clinical prevention of birth defects in non-invasive prenatal testing (NIPT) high-risk pregnant women for prenatal diagnosis, detection of fetal serious genetic defects, and clinical intervention after informed consent. Due to its advantages of high sensitivity, specificity, accuracy, and non-invasive, noninvasive prenatal test (NIPT) technology has gradually become the mainstream technology for clinical prenatal screening in recent years. Chromosome karyotype analysis technology has always been considered as the "gold standard" for diagnosing chromosomal aberrations, and is also a first-line method for prenatal diagnosis of chromosomal diseases. The main limitation of chromosome karyotype analysis is that it can not effectively detect the small structural and genetic variations of chromosomes. CNV-seq technology can not only detect conventional chromosome number and structural abnormalities, but also provide more accurate detection information for chromosome fragments from unknown sources. Microdeletion and microduplication of chromosome fragments above 5 Mb can also be detected. However, CNV-seq technology has limitations such as inability to detect balanced translocation and inversion of chromosomes, so the advantages and disadvantages of the two prenatal diagnostic techniques can complement each other. Traditional karyotype analysis combined with CNV-seq technology for prenatal diagnosis will provide more accurate and reliable prenatal diagnosis results for NIPT high-risk pregnant women and reduce birth defects. This article will briefly review the clinical application of chromosome karyotype analysis combined with CNV-seq technology in prenatal diagnosis of NIPT high-risk pregnant women, providing reference for clinical work.

Spinal cord injury disrupts the structural and functional connections between the central nervous system and the spinal cord, resulting in severe motor, sensory, and autonomic nerve dysfunctions, which seriously affect the daily living ability of patients. Transcranial magnetic stimulation is a safe, effective and non-invasive neural regulation technology, which is beneficial to the motor function recovery after spinal cord injury. This article mainly reviews transcranial magnetic stimulation in terms of assessment and treatment of motor function after spinal cord injury.

Objective: To explore the application effect of new surgical film and optical cable storage tray for operation in operating room management. Method: Random number table method from January to December 2021 to the Second Affiliated Hospital of Nanhua University after treated 280 patients with urinary calculi surgery was divided into A, B, C, D four groups, each group of 70 cases. Group A was treated with a new surgical film, group B with surgical optical cable storage tray, group C with conventional intervention, group D with a new surgical film combined with surgical optical cable storage tray. The infection status of each group, the satisfaction of patients and medical staff were compared, and the cost was calculated. Result: There was significant difference in the incidence of infection among four groups (P<0.05); the incidence of infection in group D was lower than those in group A, group B and group C, the differences were statistically significant (P<0.05). There was significant difference in the nursing satisfaction of patients among four groups (P<0.05); the nursing satisfaction of patients in group D was higher than those of group A, group B and group C, the differences were statistically significant (P<0.05). There was significant difference in the satisfaction of surgical medical staff among four groups (P<0.05); the satisfaction of surgical medical staff in group D was higher than that in group C, the differences was statistically significant (P<0.05). There was significant difference in the cost accounting among four groups (P<0.001); the economic cost in group D was lower than that in group C, but which was higher than those in group A and group B, the differences were statistically significant (P<0.05). Conclusion: The new surgical film and optical cable storage tray in the operation process of patients with urinary calculi surgery can not only reduce the infection in the operating room, but also improve the satisfaction of patients and medical staff, and reduce the cost of medical consumables.

Objective: To explore the effect of physical factor therapy on medication compliance and psychological state of elderly schizophrenic patients with shoulder, neck, waist and leg pain. Method: A total of 111 elderly schizophrenic patients with shoulder, neck, waist and leg pain who were hospitalized in Shanghai Jiading Mental Health Center from March to July 2021 were selected as the study objects. They were randomly divided into intervention group (n=55) and control group (n=56). The control group received conventional psychiatric treatment and nursing, and the intervention group received physical factor therapy on this basis. After continuous intervention for 4 weeks, the medication compliance and psychological state of the two groups were compared. Result: Before intervention, there were no significant differences between the proportion of patients with medication compliance and the proportion of patients with normal psychological state between the two groups (P>0.05). After 4 weeks of intervention, the proportion of patients with medication compliance and normal psychological state in the intervention group were higher than those in the control group, and the differences were statistically significant (P<0.05). Before intervention, there were no significant differences between the two groups in taking medication attitude score, Hamilton anxiety scale (HAMA) score and Hamilton depression scale (HAMD) score (P>0.05). After 4 weeks of intervention, the treatment attitude score of the intervention group was higher than that of the control group and before intervention, and the HAMA score and HAMD score were significantly lower than those of the control group and before intervention, the differences were statistically significant (P<0.05). Conclusion: Physical factor therapy can improve the medication compliance of elderly schizophrenic patients with shoulder, neck, waist and leg pain in hospital for a long time, and can improve their psychological state.

Objective: To explore the value of CT energy spectrum parameters in distinguishing pathological types of colorectal cancer (CRC). Method: A total of 64 CRC patients admitted to Songzi People's Hospital from February 2022 to February 2023 were selected. All patients underwent CT energy spectrum enhanced scan at 1 week before surgery. There were 36 cases of mucinous adenocarcinoma and 28 cases of tubular adenocarcinoma. The iodine concentration (IC), standardized iodine concentration (NIC), iodine Overlay value (Overlay value) and energy spectrum curve slope (λHU) of different pathological types in arterial phase and venous phase were compared, and the value of the above CT spectrum parameters in the identification of pathological types of CRC patients was analyzed. Result: IC, Overlay value, λHU in arterial phase and venous phase and NIC in arterial phase of mucinous adenocarcinoma were lower than those in tubular adenocarcinoma, and the differences were statistically significant (P<0.05), there was no significant difference between the two pathological types of NIC in venous phase (P>0.05). logistic multivariate regression analysis results showed that, arterial phase IC (OR=1.321), arterial phase NIC (OR=1.443), arterial phase Overlay value (OR=1.398), arterial phase λHU (OR=1.257), venous phase IC (OR=1.520), venous phase Overlay value (OR=1.406), venous phase λHU (OR= 1.411) were independent risk factors for tubular adenocarcinoma in CRC patients (P<0.05). Drawing receiver operating characteristic (ROC) curves showed that, the areas under the curve of arterial phase IC, arterial phase NIC, arterial phase Overlay value, arterial phase λHU, venous phase IC, venous phase Overlay value, and venous phase λHU in the identification of pathological types of CRC patients were 0.888, 0.820, 0.769, 0.832, 0.863, 0.810, and 0.723, respectively. Conclusion: The arteriovenous phase IC, Overlay value, λHU and arterial phase NIC of different pathological type in CRC patients were significant difference, and the above CT energy spectrum parameters can provide objective reference for distinguishing pathological type.

Objective: To analyze the effect of Snake Antivenins and Agkistrodon Acutus Antivenin in the treatment of trimeresurus stejnegeri bite. Method: A total of 126 patients with trimeresurus stejnegeri bite who admitted to the First Affiliated Hospital of Longyan, Fujian Medical University from January 2019 to December 2022 were included as the study objects, and they were divided into the anti-pit viper group, the anti-five-step snake group and the combined group, with 42 cases in each group. The anti-pit viper group was treated with Snake Antivenins, the anti-five-step snake group was treated with Agkistrodon Acutus Antivenin, and the combined group was treated with Snake Antivenins combined Agkistrodon Acutus Antivenin. The rehabilitation and the blood routine, inflammatory indexes and coagulation function before and after treatment among three groups were compared. Result: The swelling disappearance time of affected limb, pain disappearance time and hospitalization time in the combined group were shorter than those in the anti-pit viper group and the anti-five-step snake group, the swelling disappearance time of affected limb, pain disappearance time and hospitalization time in the anti-five-step snake group were shorter than those in the anti-pit viper group, and the differences were statistically significant (P<0.05). After treatment, the erythrocyte, hemoglobin, white blood cell count, calcitonin, C-reactive protein, serum amyloid A, prothrombin time, thrombin time and fibrinogen in three groups were significantly improved compared with those before treatment, and the differences were statistically significant (P<0.05), the erythrocyte, hemoglobin and fibrinogen in the combined group were higher than those in the anti-pit viper group and the anti-five-step snake group, the white blood cell count, calcitonin, C-reactive protein and serum amyloid A were lower than those in the anti-pit viper group and the anti-five-step snake group, the prothrombin time and thrombin time were shorter than those in the anti-pit viper group and the anti-five-step snake group, and the erythrocyte, hemoglobin and fibrinogen in the anti-five-step snake group were higher than those in the anti-pit viper group, the white blood cell count, calcitonin, C-reactive protein and serum amyloid A in the anti-five-step snake group were lower than those in the anti-pit viper group, and the prothrombin time and thrombin time in the anti-five-step snake group were shorter than those in the anti-pit viper group, the differences were statistically significant (P<0.05). Conclusion: Both Snake Antivenins and Agkistrodon Acutus Antivenin can effectively improve the blood routine, inflammatory response and coagulation function of patients bitten by trimeresurus stejnegeri, the improvement effect of Agkistrodon Acutus Antivenin is better than that of Snake Antivenins, the combination of two can further enhance the clinical efficacy, accelerate the rehabilitation process, and shorten the time of symptom improvement and hospitalization.

Objective: To investigate the effect of Esketamine combined with Remifentanil and Propofol anaesthesia on pain levels and postoperative awakening in patients undergoing ureteral stent removal. Method: A total of 95 patients who underwent ureteral stent removal in Hai’an Hospital of Traditional Chinese Medicine from June 2022 to June 2023 were selected. Random number table method was used to divide the control group (n=47) and the study group (n=48). The control group was given Remifentanil+Propofol anesthesia, and the study group was given Remifentanil+Propofol+Esketamine anesthesia. Anesthesia effect, pain degree, quality of recovery, cognitive function and incidence of adverse reactions were analyzed and compared between the two groups. Result: The total excellent and good rate of anesthesia in the study group was 95.83%, which was significantly higher than 80.85% in the control group, and the difference was statistically significant (P<0.05). The visual analogue scale (VAS) score of the study group was significantly lower than that of the control group, and the recovery time was earlier than that of the control group, the differences were statistically significant (P<0.05). At 1 h and 3 h after operation, the score of mini-mental state examination (MMSE) in the study group was significantly higher than that in the control group, and the difference was statistically significant (P<0.05). The total incidence of adverse reactions in the study group was 8.33%, which lower than 14.89% in the control group, but there was no significant difference between the two groups (P>0.05). Conclusion: Esketamine combined with Remifentanil and Propofol can significantly reduce the pain of patients undergoing ureteral stent removal, reduce cognitive impairment, shorten postoperative recovery time, and have a high safety.

Objective: To observe and analyze the effect of Sertraline combined with Tandospirone in the treatment of adolescent depression patients with non-suicide self-injury behavior (NSSI). Method: A total of 72 adolescent depression patients with NSSI who treated in Fujian Province Fuzhou Neuropsychiatric Hospital from October 2022 to January 2023 were selected as the research objects, the patients were divided into the control group and the observation group by random number table method according to the order of hospitalization, with 36 cases in each group. Both groups were treated with Sertraline Hydrochloride Tablets, and the observation group was treated with Tandospirone Citrate Capsules. The clinical efficacy, negative emotions [Hamilton depression scale-17 item (HAMD-17) score, Hamilton anxiety scale (HAMA) score], impulsive behavior [impulse scale (BIS-11)], NSSI behavior and adverse reactions were compared between two groups. Result: The total effective rate in the observation group was higher than that in the control group, the difference was statistically significant (P<0.05). There were no statistically significant differences in HAMD-17 score and HAMA score between two groups before treatment (P>0.05); after treatment, the HAMD-17 score and HAMA score of two groups were lower than those before treatment, which in the observation group were lower than those in the control group, the differences were statistically significant (P<0.05). Before treatment, there were no significant difference in the attention, exercise, lack of planning and total scores between two groups (P>0.05); after treatment, the attention, exercise, lack of planning and total scores of two groups were lowere than those before treatment, and which in the observation group were lower than the control group, the differences were statistically significant (P<0.05). Before treatment, there were no significant difference in the frequency of occurrence of NSSI behavior between two groups (P>0.05); after 2 weeks and 4 weeks of treatment, the frequency of occurrence of NSSI behavior in two groups were less than those before treatment, and which in the observation group were less than those in the control group, the differences were statistically significant (P<0.05). There was no significant difference in the incidence of adverse reactions between two groups (P>0.05). Conclusion: Sertraline combined with Tandospirone in the treatment of adolescent depression patients with NSSI can significantly improve anxiety and depression, improve impulsive behavior and NISSI behavior, and will not increase adverse reactions.

Objective: To analyze the ABO difficult blood type identification and reasons for discrepancy between positive and negative typing. Method: From January 2021 to May 2023, 92 ABO difficult blood type samples were selected from Xinglin Branch, the First Affiliated Hospital of Xiamen University. All blood samples was determined by saline test tube method for blood type positive and negative typing, and the blood type was further determined by polybrene method and absorption and release test. The coincidence rate of blood type positive and negative typing measured by three methods was counted, and the reasons for non-conformity of positive and negative typing of saline test tube method were analyzed, and ABO blood type identification was completed. Result: The detection results of 92 blood samples showed that the coincidence rates of blood type positive and negative typing were 44.57% (41/92), 51.09% (47/92), 77.17% (71/92) respectively by saline test tube method, saline test tube method + polybrene method, saline test tube method + polybrene method + absorption and release test. The main reasons for non-conformity of positive and negative typing in saline test tube method were AB subtype, weak B antigen, weak A antigen, no anti-B antibody type A and no antibody type O. Among the 92 ABO difficult blood types, 29 cases were subtype A, 50 cases were subtype B, and 13 cases were subtype AB. Conclusion: The positive and negative typing of ABO difficult blood type by saline test tube method + polybrene method + absorption and release test can improve the accuracy of blood type identification in clinic.

Epilepsy is a chronic brain disease caused by abnormal discharge of brain neurons, which is characterized by recurrent, episodic and transient central nervous system dysfunction, which can not only induce migraines, anxiety disorders and other diseases, but also lead to psychological and social dysfunction in patients, seriously affecting the physical and mental health of patients. The pathogenic factors of epilepsy are many and complex, but its pathogenic mechanism has not been fully clarified, which may be related to abnormal discharge of brain neurons and abnormal activity of cerebral cortex. The treatment period of epilepsy is long and difficult, and the effect is not good for drug-resistant epilepsy and generalized tonic epilepsy. Nitric oxide (NO), as a diatomic gas, widely exists in the human body and can be involved in regulating the basic activities of the human body. NO is obviously correlated with epilepsy and has a bidirectional regulatory effect, so NO can be used to treat epilepsy, but adverse reactions may occur in the treatment process, and its clinical effect needs to be further optimized. This article reviewed the relationship between NO and the pathogenesis of epilepsy in order to provide new ideas for clinical diagnosis and treatment.

Objective: To analyze of the influencing factors of the effect of flexible ureteroscopic lithotripsy (F-URS) in the treatment of residual stones after extracorporeal shock wave lithotripsy (ESWL) of lower renal calyx. Method: A total of 98 patients with residual stones after ESWL of lower renal calyx who admitted to the Department of Urology in Wuxi No.8 People's Hospital were selected as the research objects, they were divided into the good prognosis group (83 cases) and the poor prognosis group (15 cases) according to the stone clearance. The correlation between residual stone free rates (SFR) and influencing factors were analyzed, and the influencing factors of residual stone clearance effect were analyzed by univariate and multivariate analysis. Result: The cases of stone diameter >20 mm number and high stone density in the poor prognosis group were more than those in the good prognosis group, the stone volume, stone CT value, stone width, stone length and stone morphological parameters were higher than those in the good prognosis group, and the differences were statistically significant (P<0.05); there were no significant differences in the number of stones, intraoperative blood loss, postoperative hospital stay, intraoperative lithotripsy success rate and stone inclusion ratio between two groups (P>0.05). The stone diameter, stone density, stone volume, stone CT value, stone width, stone length and stone morphological parameters were negatively correlated with the stone clearance effect (P<0.05). Multivariate logistic regression analysis showed that stone diameter, stone density, stone volume, stone CT value, stone width, stone length and stone morphological parameters were independent influencing factors of residual stone removal effect (P<0.05). The receiver operating characteristic (ROC) curve showed that the area under the curve (AUC) was 0.965 (0.929, 0.999), the sensitivity was 0.866 (0.775, 0.932), and the specificity was 0.937 (0.681, 0.998), the difference was statistically significant (P<0.05). Conclusion: The stone diameter, stone density, stone volume, stone CT value, stone width, stone length and stone morphological parameters were higher than those in the good prognosis group are negatively correlated with the effect of stone clearance in patients with residual stones after ESWL of lower renal calyx by F-URS, and these factors are also independent influencing factors of residual stone clearance.

Objective: To analyze the effects of acupoint application of traditional Chinese medicine combined with enhanced recovery after surgery (ERAS) on postoperative rehabilitation, complication risk and nursing satisfaction in patients with colorectal cancer. Method: A total of 300 postoperative patients with colorectal cancer treated in the First People's Hospital of Foshan City from January 2021 to December 2023 were selected and divided into observation group and control group according to random drawing method, with 150 cases in each group. The control group was treated with ERAS nursing, and the observation group was treated with acupoint application of traditional Chinese medicine combined with ERAS nursing. The intervention effect of the two groups were compared. Result: The exhaust time, bowel sound recovery time and defecation time in the observation group were shorter than those in the control group, and the differences were statistically significant (P<0.05). One week after operation, the traditional Chinese medicine syndrome score of the observation group were lower than those of the control group, the differences were statistically significant (P<0.05); and the hospital stay of the observation group was shorter than that of the control group, the difference was statistically significant (P<0.05). The incidence of postoperative complications in the observation group was lower than that in the control group, and the difference was statistically significant (P<0.05). The total nursing satisfaction of the observation group was higher than that of the control group, and the difference was statistically significant (P<0.05). Conclusion: The application of acupoint application of traditional Chinese medicine combined with ERAS concept has achieved a good effect in patients with colorectal cancer after operation.

Objective: To explore the application effect of early warning management intervention based on nurses' crisis awareness in stroke patients with hemiplegia. Method: A total of 116 patients with stroke hemiplegia who received treatment in Jiangsu Rongjun Hospital from January 2020 to June 2022 were selected as the study objects and randomly divided into two groups, with 58 patients in each group. The control group was given routine intervention, and the observation group was given early warning management intervention based on nurses' crisis awareness. The incidence of deep vein thrombosis (DVT) and lower limb swelling, limb motor function, negative emotion and quality of life were compared between the two groups. Result: After 1 week of intervention, the incidence of DVT and lower limb swelling in the observation group were significantly lower than those in the control group, the differences were statistically significant (P<0.05). After 1 week of intervention, the upper limb score, lower limb score and total score of the observation group were significantly higher than those of the control group, and the differences were statistically significant (P<0.05). After 1 week of intervention, self-rating anxiety scale (SAS) scores and self-rating depression scale (SDS) scores in the observation group were significantly lower than those in the control group, and the differences were statistically significant (P<0.05). The stroke specific quality of life scale (SS-QOL) score of the observation group was significantly higher than that of the control group after 1 week of intervention, and the difference was statistically significant (P<0.05). Conclusion: The application of early warning management intervention based on nurses' crisis awareness in stroke patients with hemiplegia can reduce the adverse events such as DVT and lower limb swelling, improve their limb motor function, and significantly relieve patients' negative emotions and promote the improvement of their quality of life.

The function of ovarian reserve reflects the potential of female reproductive endocrinology, and the decrease of ovarian reserve function is a dangerous signal of female fertility decline. Teacher Bi Ying believed that kidney essence deficiency was the main pathogenesis of the disease, and the leucorrhea in the late menstrual period and the geumsa leucorrhea in the intermenstrual period directly reflect the development of follicles and ovulation, and indirectly reflected the function of the ovary. The cycle change of femal leucorrhea is the same as menstruation under the premise of Yin and Yang mutual root. Based on the idea of "seeking Yin from Yang" and "using Yang to convert Yin", warming acupuncture and moxibustion in uterine acupoint can make Yin get Yang rising and the source is inexhaustible, make Qi and blood flow, Yin and Yang in equilibrium, and thus achieve the purpose of improving ovarian function and regulating menstruation seeds.

Objective: To explore the shunt value of gene methylation testing combined with high-risk HPV testing for cervical cancer screening. Method: The clinical data of 80 women who underwent opportunistic screening at the Cervical Disease Diagnosis and Treatment Center of Quanzhou First Hospital from October 2020 to October 2022 was collected, all of whom underwent gene methylation testing, high-risk HPV testing, and cervical pathological biopsy under colposcopy. Using the results of cervical pathological biopsy as the gold standard, and the consistency of gene methylation testing, high-risk HPV testing alone and in combination with the gold standard, and the diagnostic efficacy of cervical cancer, and the screening diversion of cervical cancer were compared. Result: Among the 80 cases of cervical cancer screening, a total 23 cases were positive in cervical pathological biopsy results, accounting for 28.75% (23/80), and 57 cases were negative, accounting for 71.25% (57/80). Among the 23 positive cases, cervical squamous cell carcinoma (SCC) accounted for 43.48% (10/23), low-grade squamous intraepithelial lesions (LSIL) accounted for 39.13% (9/23), and high grade squamous intraepithelial lesions (HSIL) accounted for 17.39% (4/23). There were 16 cases diagnosed with cervical cancer by gene methylation testing, and 15 cases diagnosed with cervical cancer by high-risk HPV testing, which showed poor consistency with the gold standard (Kappa=0.372, 0.357); there were 19 cases diagnosed with cervical cancer by combining the two tests, which showed good consistency with the gold standard (Kappa=0.784). The specificity, accuracy, and negative predictive value of gene methylation testing combined with high-risk HPV testing for screening cervical cancer were significantly higher than those of gene methylation testing and high-risk HPV testing alone, the differences were statistically significant (P<0.05). There were no statistically significant differences in the sensitivity and positive predictive value of the combined test compared to the two tests alone (P>0.05). The positive testing rate of SCC in combination testing was higher than that in individual testing, and the referral rate of colposcopy was lower than that in individual testing, the differences were statistically significant (P<0.05). There were no statistically significant differences in the positive testing rates of HSIL and LSIL detected by gene methylation testing, high-risk HPV testing and combination testing (P>0.05). Conclusion: The combination of gene methylation testing and high-risk HPV testing has high value in the diagnosis of cervical cancer. It can improve the positive diagnosis rate of cervical cancer and reduce the referral rate of colposcopy through shunt testing in cytological abnormalities.

The intestinal flora is large in number and variety, which is an important microorganism that determines the level of human health. Maintaining the diversity and abundance of intestinal flora can largely avoid the occurrence of a series of diseases such as intestinal inflammation. This article discusses the effects of phages on human intestinal flora and intestinal inflammation. Based on the relevant studies of many scholars, this article reviews the effects of intestinal phages on intestinal flora and intestinal inflammation, and proposes the use of phages to adjust the function of intestinal flora to treat intestinal inflammation, which provides a new direction for the regulation of intestinal flora and the treatment of intestinal inflammation in the future. We hope to provide some references for readers in the research and work in related fields.

Objective: To explore the effect of three-dimensional conformal radiotherapy combined with Lianqi Capsules on traditional Chinese medicine symptom scores, tumor marker levels and immune function of patients with middle and advanced esophageal cancer. Method: A total of 80 patients with middle and advanced esophageal cancer admitted to Putian 95 Hospital from January 2019 to November 2022 were selected as the study objects, they were divided into two groups according to random number table method, with 40 patients in each group. The control group received three-dimensional conformal radiotherapy, and the observation group was additionally given Lianqi Capsules. Traditional Chinese medicine symptom scores, tumor markers [squamous cell carcinoma antigen (SCC-Ag), carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA21-1)] and immune function [T lymphocyte subsets (CD4+, CD8+, CD4+/CD8+)] were compared between the two groups. Result: After 6 weeks of treatment, the scores of traditional Chinese medicine symptom in both groups were lower than those before treatment, those in the observation group were lower than those in the control group, the differences were statistically significant (P<0.05). After 6 weeks of treatment, the levels of SCC-Ag, CEA and CYFRA21-1 in both groups were lower than those before treatment, while those in the observation group were lower than those in the control group, the differences were statistically significant (P<0.05). After 6 weeks of treatment, CD4+ and CD4+/CD8+ in both groups were lower than those before treatment, those in the observation group were higher than those in the control group; CD8+ in both groups were higher than those before treatment, but that in the observation group was lower than that in the control group, the differences were statistically significant (P<0.05). Conclusion: Three-dimensional conformal radiotherapy combined with Lianqi Capsules of patients with advanced esophageal cancer can relieve the traditional Chinese medicine symptom scores, reduce the level of tumor markers and improve the immune function.

Breast cancer is the cancer with the highest incidence rate at present, the recurrence and metastasis of breast cancer is one of the unsolved clinical problems. With the development of traditional Chinese medicine, traditional Chinese medicine has played an irreplaceable role in the treatment of recurrent and metastatic breast cancer, and has achieved good clinical efficacy. Through literature research, this paper discussed the clinical research progress of traditional Chinese medicine in the recognition, pathogenesis and prevention of recurrent metastatic breast cancer, and systematically evaluated the advantages of traditional Chinese medicine and its shortcomings that still need to be improved.

Objective: To explore the therapeutic effect and mechanism of Roxadustat on uremic maintenance hemodialysis patients with Renal Anemia. Method: A total of 64 uremic maintenance hemodialysis patients with renal anemia who treated in Suzhou Ninth People's Hospital from January 2019 to April 2022 were randomly divided into the experimental group and the control group, 32 cases in each group. The control group was treated with Exogenous Erythropoietin, and the experimental group was treated with Roxadustat, two groups were treated for 2 months. The serum hemoglobin (Hb), erythropoietin (EPO) anemia index, interleukin-6 (IL-6), ferritin metabolism level, C-reactive protein (CRP) and clinical efficacy of two groups were compared. Result: Before treatment, there were no significant differences in EPO and Hb between two groups (P>0.05); after treatment, EPO and Hb in two groups increased compared with those before treatment, and the experimental group were higher than those in the control group, the differences were statistically significant (P<0.05). Before treatment, there were no significant differences in the levels of IL-6 and ferritin between two groups (P>0.05); after treatment, the levels of ferritin and IL-6 in two groups decreased compared with those before treatment, and the experimental group were lower than those in the control group, the differences were statistically significant (P<0.05). Before treatment, there was no significant difference in CRP levels between two groups (P>0.05); after treatment, the CRP levels in two groups decreased compared with those before treatment, and the experimental group was lower than that in the control group, the differences were statistically significant (P<0.05). The total effective rate of the experimental group (93.75%) was higher than that of the control group (68.75%), the difference was statistically significant (P<0.05). Conclusion: Roxadustat in the treatment of uremic maintenance hemodialysis patients with renal anemia is conducive to improve anemia, promote IL-6 and ferritin metabolism, reduce inflammatory reaction, and improve the effect.

Objective: To analyse the diagnostic value of combining tumor markers carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA199), carbohydrate antigen 242 (CA242) and carbohydrate antigen 724 (CA724) in early gastric cancer. Method: From June 2021 to June 2023, 56 suspected early gastric cancer patients admitted to Clinical Oncology School of Fujian Medical University were selected as the observation group. In addition, 40 patients with benign stomach diseases admitted to in the same period were selected as the control group. The levels of the tumor markers CEA, CA199, CA242 and CA724 were compared between the two groups, and the diagnostic value of the above four tumor markers were tested alone and in combination was analyzed. Result: The levels of CEA, CA199, CA242 and CA724 in observation group were significantly higher than those in control group, the differences were statistically significant (P<0.05). Among the 56 suspected early gastric cancer patients in the observation group, there were 48 positive cases (25 papillary adenocarcinoma cases, 23 tubular adenocarcinoma cases) and 8 negative cases (5 gastric ulcers cases, 3 chronic gastritis cases). The results showed that the sensitivity and accuracy of the combined detection of CEA, CA199, CA242 and CA724 were 93.75% and 92.86%, which were higher than the sensitivity and accuracy of the four separate tests, and the differences were statistically significant (P<0.05). There was no significant difference between the specificity of the combined test and that of the four separate tests (P>0.05). Conclusion: The combined detection of tumor markers CEA, CA199, CA242 and CA724 has high diagnostic value, which is significantly superior to each single detection, and can provide an important theoretical reference for the rapid screening and diagnosis of early gastric cancer.

Objective: To investigate the correlation among hepatic transient elastography index, spleen thickness, portal vein inner diameter, fibrosis 4 index (FIB4), aspartate aminotransferase to platelet ratio index (APRI) and the degree of esophageal varices in liver cirrhosis. Method: From December 2020 to February 2022, 40 patients with severe esophageal varices in liver cirrhosis admitted to the Second People's Hospital of Tianjin were selected as group A, 40 patients with moderate esophageal varices in liver cirrhosis were selected as group B, 40 patients with mild esophageal varices in liver cirrhosis were selected as group C, and 40 patients without esophageal varices in liver cirrhosis were selected as group D. Hepatic transient elastography, alanine aminotransferase (ALT), aspartate aminotransferase (AST), platelet (PLT) detection were performed in all the four groups, and FIB4 and APRI were calculated. The hepatic transient elastometry indexes, spleen thickness, portal vein inner diameter, FIB4 and APRI of four groups were compared. Spearman rank correlation was used to analyze the relationship among the above detection indexes and the severity of esophageal varices in liver cirrhosis. Result: The liver stiffness value, spleen stiffness value, spleen thickness and portal vein inner diameter, FIB4 and APRI in group A, group B and group C were significantly higher than those in group D, the liver stiffness value, spleen stiffness value, spleen thickness and portal vein inner diameter, FIB4 and APRI in group A and group B were significantly higher than those in group C, the liver stiffness value, spleen stiffness value, spleen thickness and portal vein inner diameter, FIB4 and APRI of group A were higher than those of group B, and the differences were statistically significant (P<0.05). Spearman rank correlation analysis showed that liver stiffness value, spleen stiffness value, spleen thickness, portal vein inner diameter, FIB4, APRI were positively correlated with the degree of esophageal varices in liver cirrhosis (P<0.05). Conclusion: The expression of hepatic transient elastography index, spleen thickness, portal vein inner diameter and FIB4, APRI of patients with liver cirrhosis esophageal varices are significantly abnormal, and the above indicators are closely related to the degree of esophageal varices, so their detection value in the patients with liver cirrhosis esophageal varices is high.

Objective: To analyze the application effect of different doses of Folic Acid in obese polycystic ovary syndrome (PCOS) patients with hyperhomocysteinemia (HHcy). Method: A total of 88 obese PCOS patients with HHcy who were treated in the Outpatient Department of Nanhai District Maternal and Child Health Hospital of Foshan from February 2020 to February 2022 were retrospectively selected. According to the different oral dose of Folic Acid, they were divided into low dose group and high dose group, 44 cases in each group. The low dose group was given 1 mg daily oral Folic Acid, and the high dose group was given 3.5 mg daily oral Folic Acid. The glucose metabolism index, lipid metabolism index, inflammatory index before treatment and after 12 weeks of treatment and adverse reactions were compared between the two groups. Result: After 12 weeks of treatment, fasting plasma glucose (FPG), insulin resistance index (HOMA-IR), total cholesterol (TC), fasting insulin (FINS), high-sensitivity C-reactive protein (hs-CRP), homocysteine (Hcy) in two groups and low density lipoprotein cholesterol (LDL-C) in the high dose group decreased compared with that before treatment, FPG, HOMA-IR, TC, FINS, hs-CRP, Hcy and LDL-C in the high dose group were significantly lower than those in the low dose group, the differences were statistically significant (P<0.05). There were no shedding cases and no obvious adverse reactions during treatment in both groups. Conclusion: Short-term oral high dose (3.5 mg/d) Folic Acid treatment can improve the glycolipid metabolism, body micro inflammation status and reduce the level of Hcy in obese PCOS patients with HHcy.

Objective: To explore the status quo of postoperative autonomic perception in patients with endometrial cancer, and to analyze the related influencing factors. Method: A total of 72 patients with endometrial cancer admitted to Affiliated Hospital of Putian University from April 2020 to April 2022 were taken as the research object. The simplified Chinese version of the PEA scale (PEAS-SCV) was used to assess the level of patients' autonomic perception, a baseline data questionnaire was designed, and the clinical data of all patients were collected in detail. The differences of patients' relevant data were investigated and compared, and the possible factors were included, multivariate linear regression was used to analyze the influencing factors of postoperative autonomic perception in patients with endometrial cancer. Result: The PEAS-SCV score range of 72 patients with endometrial cancer was 50-71 points, with an average score of (60.50±5.29) points. There were statistically significant differences in endometrial cancer patients with different education level, social support, hope level and family care (P<0.05). The results of multiple linear regression analysis showed that education level, social support, hope level and family care were the influencing factors of the status of autonomic perception of endometrial cancer patients after surgery (P<0.05). Conclusion: The level of self perception of endometrial cancer patients after surgery is slightly low. Education, social support, hope level and family care are the influencing factors of the status of self perception of endometrial cancer patients after surgery.

Objective: To observe the effect of single-port thoracoscopic radical resection of lung cancer on perioperative indicators and survival in patients with early non-small cell lung cancer (NSCLC). Method: The clinical data of 40 patients with NSCLC who underwent single-port thoracoscopic radical resection of lung cancer in Qiandongnan People's Hospital from July 2018 to November 2021 were retrospectively collected and included in group A. In addition, the clinical data of 40 patients with NSCLC who underwent three-port thoracoscopic radical resection of lung cancer in our hospital during the same period were collected and included in group B. The surgical indexes, postoperative pain [visual analogue scale (VAS)], stress indexes [cortisol (Cor), norepinephrine (NE), angiotensin Ⅱ (Ang Ⅱ)], postoperative complications and survival were compared between two groups. Result: The operation time of group A was longer than that of group B, the chest tube indwelling time and hospitalization time were shorter than those of group B, and the differences were statistically significant (P<0.05); the VAS scores of group A at 12 h, 24 h and 48 h after operation were lower than those of group B, and the differences were statistically significant (P<0.05); at 1 d after operation, Cor, NE and Ang Ⅱ in two groups increased, but those in group A were lower than those in group B, and the differences were statistically significant (P<0.05); the incidence of postoperative complications in group B was higher than that in group A, the difference was statistically significant (P<0.05); there were no significant differences in the 1-year and 3-year survival rates between two groups (P>0.05). Conclusion: Single-port thoracoscopic radical resection of lung cancer in the treatment of early NSCLC patients has less trauma, which can reduce postoperative stress response, relieve postoperative pain, facilitate postoperative recovery, and shorten hospitalization time, and the survival rate is high.

Acute pulmonary embolism (APE) is a common and potentially fatal thrombotic disease, while atrial fibrillation (AF) is a common arrhythmia, whose coexistence with pulmonary embolism (PE) has received increasing attention in recent years. Both APE and AF are associated with significant morbidity and mortality, respectively, and patients with APE with AF have poorer clinical outcomes and higher hospitalization and long-term mortality. Its mechanism is relatively complex, which is related to a variety of factors, and both of them interact to increase the risk of hospitalization. Therefore, it is particularly important to understand the relationship between APE and AF. On the one hand, AF may increase the risk of APE and affect the short-term and long-term survival rate of patients; on the other hand, APE may cause cardiac dysfunction and thus induce AF. This article described the research progress of the correlation between APE and AF.

Objective: To explore the clinical effect of Dupilumab combined with Desonide Cream in the treatment of severe atopic dermatitis in children. Method: A total of 78 children with severe atopic dermatitis who treated in Quanzhou First Hospital from February 2020 to February 2022 were selected as the study subjects, they were divided into the control group and the experimental group according to the random number table method, 39 cases in each group. Both groups were treated with Desonide Cream, and the experimental group was treated with Dupilumab on the basis. The treatment effect, treatment safety, clinical symptom relief time, eosinophil level and children's skin lesion symptom score were compared between two groups. Result: The total effective rate of the experimental group was higher than that of the control group, the remission times of eczema-like rash, dry skin and pruritus were earlier than those of the control group, the eosinophil level and eczema area and severity index (EASI) score after treatment were lower than those of the control group, the differences were statistically significant (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion: The treatment of children with severe atopic dermatitis treated with Dupilumab combined with Desonide Cream has achieved better clinical results, which can reduce eosinophil level and EASI score, significantly shorten the remission time of clinical symptoms. Moreover, adverse reactions are not significantly increased during the process.

CXC chemokine 8 (CXCL8) is a small molecule secreted inflammatory cytokine with chemotaxis function, belonging to the CXC chemokine family, also known as interleukin 8 (IL-8). In recent years, research has found that various cells in the body, such as monocytes, macrophages, endothelial cells, keratinocytes, tumor cells etc, have the ability to secrete CXCL8. After binding to CXCL8 and its corresponding receptor CXCR1, in addition to chemotaxis and inducing degranulation of neutrophils, CXCL8 can also affect the proliferation, metastasis and invasion of tumor cells by promoting angiogenesis, and play an important role in the occurrence, development and metastasis of tumors. In this paper, we summarized the related description of CXCL8 and its receptor, the message axis of CXCL8/CXCR1, and the role of CXCL8 and its receptor in tumor, hoping to provide reference for related studies.

Primary Sj.gren's syndrome (PSS) is a chronic inflammatory connective tissue disease characterized by invading exocrine gland, it is more common in female, it can involve multiple organ systems, such as respiratory system, blood system, nervous system, skin, muscle, bone, etc, its prominent symptoms are dry mouth, dry eyes. PSS complicated with depression is more common, the occurrence of depression seriously affects the physical and mental health and quality of life of PSS patients, and brings a very heavy burden to the family and society, it is more and more attention, with the deepening of the research, the pathogenesis of depression in PSS patients is complex and diverse. This paper comprehensively analyzed the pathogenesis of PSS complicated with depression from the aspects of somatic-social-psychological, immune system, neuroendocrine system and cognitive function, in order to improve the treatment methods and quality of life of PSS complicated with depression patients.

Objective: To investigate the changes of electrocardiogram characteristic and heart rate variability (HRV) indexes in patients with pre-diabetes mellitus (DM) (pre-DM) and the predictive value for cardiovascular adverse events. Method: A total of 118 pre-DM patients admitted to Youxi County General Hospital from January 2019 to December 2020 were selected as the observation group, and 83 healthy physical examination personnel in our hospital during the same period were selected as the control group. HRV indexes of the two groups were detected, electrocardiogram characteristics of the two groups were analyzed, and 1-year follow-up investigation was carried out in the observation group. The HRV index and electrocardiogram characteristics of the two groups were compared. The occurence condition of cardiovascular adverse events in the observation group was analyzed, and the R-R interval standard deviation in all sinus beats (SDNN), standard deviation of R-R interval mean (SDANN), total spectrum, Q-T interval dispersion (QTd) and corrected QTd value (QTcd) were compared between patients with and without cardiovascular adverse events. The influencing factors of the occurence of cardiovascular adverse events in pre-DM patients was analyzed multifactorially. The predictive value of SDANN, QTd and QTcd for cardiovascular adverse events was analyzed. Result: The SDNN, SDANN and total spectrum in observation group were lower than those in control group, and QTd and QTcd were greater than those in control group, the differences were statistically significant (P<0.05). After 1-year follow-up, 21 patients in the observation group had cardiovascular adverse events, the incidence was 17.80% (21/118). In the observation group, the SDANN of patients with cardiovascular adverse events was lower than that of patients without cardiovascular adverse events, and the QTd and QTcd were greater than those of patients without cardiovascular adverse events, the difference was statistically significant (P<0.05). Prolonged QTd and QTcd were independent risk factors for cardiovascular adverse events in pre-DM patients, and increased SDANN was a protective factor (P<0.05). The AUC predicted by SDANN, QTd and QTcd for cardiovascular adverse events in pre-DM patients was 0.956, 0.679 and 0.714 respectively, and the AUC of joint prediction was 0.961. Conclusion: The SDANN decreased and QTd and QTcd prolonged in pre-DM patients, and the combination of the three predicted cardiovascular adverse events with the highest AUC, which is conducive to clinical monitoring and reducing the occurrence of cardiovascular adverse events in pre-DM patients.