Objective: To explore the significance of serum cytokeratin 18-M30 fragment (CK18-M30) and cytokeratin 18-M65 fragment (CK18-M65) levels in screening of fatty liver in physical examination population, and analysis of risk factors for fatty liver. Method: A total of 147 physical examiners from July 2020 to June 2022 in Taicang First People's Hospital were selected as the subjects of this study. The detection of fatty liver in physical examiners was recorded, and they were divided into fatty liver group (n=38) and non fatty liver group (n=109) according to whether they had fatty liver. The expression levels of CK18-M30 and CK18-M65 in serum of the two groups were compared, the factors influencing fatty liver were analyzed by univariate and multivariate logistic regression, and the application value of their expression levels in the diagnosis of fatty liver was analyzed with receiver operating characteristic (ROC). Result: Among 147 examiners, a total of 38 patients with fatty liver were detected, with a detection rate of 25.85% (38/147). The expression levels of CK18-M30 and CK18-M65 in the serum of the fatty liver group were higher than those of the non fatty liver group, the differences were statistically significant (P<0.05). The results of univariate analysis showed that there were no significant differences in gender, age, and the levels of tumor necrosis factor-α (TNF-α), C-reactive protein (CRP) and aspartate aminotransferase (AST) between the two groups (P>0.05). The body mass index (BMI), waist circumference, the proportion of diabetes, hypertension, hyperlipidemia and alanine aminotransferase (ALT) levels in the fatty liver group were significantly higher than those in the non fatty liver group, the differences were statistically significant (P<0.05). Multivariate logistic regression analysis showed that high BMI (OR=1.874), high waist circumference (OR=2.114), combined diabetes (OR=2.257), high CK18-M30 (OR=2.577), and high CK18-M65 (OR=1.974) were independent risk factors for fatty liver (P<0.05). The results of ROC analysis showed that the area under the curve (AUC) of CK18-M30 and CK18-M65 in the diagnosis of fatty liver was 0.885 (95%CI: 0.822, 0.948), significantly higher than 0.735 (95%CI: 0.636, 0.834) of CK18-M30 alone and 0.778 (95%CI: 0.704, 0.853) of CK18-M65 alone. Conclusion: High BMI, High waist circumference, combined diabetes, high CK18-M30 and high CK18-M65 are independent risk factors for fatty liver. The levels of CK18-M30 and CK18-M65 show abnormally high expression in the serum of patients with fatty liver, which has good clinical diagnostic effect in fatty liver, and can be used as effective indicators for screening fatty liver in the physical examination population.

Migraine is a common chronic neurovascular disease in clinical practice, with repeated attacks, stubborn and difficult to heal as the main characteristics, to the patient's physical and psychological caused significant distress, greatly affecting the quality of life of patients. In recent years, the in-depth study of intestinal microecology theory has found that intestinal flora can participate in the occurrence and development of migraine through various ways. Based on the theory of "microbial-gut-brain axis", this paper discusses the four aspects of meridians, Qi and blood, emotions, phlegm turbidity, so as to provide the basis for the treatment of migraine by spleen and stomach in traditional Chinese medicine.

Objective: To analyze the influence of hospital to family seamless model nursing on the stigma and negative psychology of patients undergoing enterostomy. Method: A total of 80 patients undergoing enterostomy admitted to the Department of General Surgery of the First Affiliated Hospital of Xiamen University from January 2021 to June 2022 were selected as the study objects. They were divided into control group and observation group according to random number table method, with 40 cases in each group. The control group was given routine nursing, and the observation group was given hospital to family seamless model nursing on the basis of control group. Stigma, negative psychology before and after nursing and gastrointestinal function recovery time were compared between the two groups. Result: After nursing, the scores of social exclusion, intrinsic shame and social isolation in the observation group were lower than those in the control group, and the differences were statistically significant (P<0.05). After nursing, depression score and anxiety score in the observation group were lower than those in the control group, and the differences were statistically significant (P<0.05). The first feeding time, first ostomy defecation time and first ostomy exhaust time in the observation group were significantly earlier than those in the control group, and the differences were statistically significant (P<0.05). Conclusion: The hospital to family seamless model nursing can effectively reduce the sense of shame and negative psychological emotions of patients, and is more conducive to promoting the recovery of gastrointestinal function of patients.

　　Constipation is a common clinical symptom, which often leads to anorectal diseases, colorectal cancer, acute myocardial infarction, cerebrovascular accidents and other diseases. It affects the daily life of patients and causes economic and social burden. The unique "holistic concept" and "syndrome differentiation and treatment" theoretical system of traditional Chinese medicine has advantages in the treatment of constipation. In clinical practice, chronic constipation is common in the form of asthenia constipation, mainly in the form of Qi deficiency and Yin deficiency, and mostly mixed with each other. Therefore, this paper briefly describes the methods of treating constipation with deficiency of both Qi and Yin in recent years, with a view to providing inspiration or improving the deficiency of the treatment of constipation with deficiency of both Qi and Yin.

Objective: To compare the differences in clinical pharmacodynamic characteristics between Ciprofol and Propofol. Method: From July to October 2021, 80 patients underwent internal fixation of distal upper limb fractures in the Affiliated Hospital of Panzhihua University were randomly divided into Ciprofol group and Propofol group, 40 cases in each group. After the completion of ultrasound-guided brachial plexus block, two groups were intravenously pumped with 2 mg/kg Propofol and 0.4 mg/kg Ciprofol for 1 min. The mean arterial pressure (MAP), heart rate (HR), blood oxygen saturation (SpO₂) and bispectral index (BIS) were recorded and compared between two groups before administration (T0) and 1 min (T1), 2 min (T2), 3 min (T3), 4 min (T4), 5 min (T5) after the start of pumping drugs; the induction time, consciousness recovery time and orientation recovery time of two groups were observed; in addition, the injection pain score and adverse reactions were compared between the two groups. Result: There were significant differences in MAP, HR, SpO₂ and BIS at different time points between two groups (P<0.05); MAP and SpO₂ at T1, T2, T3, T4, T5 in Propofol group were lower than those at T0, the differences were statistically significant (P<0.05); MAP at T1 and T2 in Ciprofol group were lower than those at T0, and SpO₂ at T1, T2, T3, T4 were lower than those at T0, the differences were statistically significant (P<0.05); MAP and SpO₂ at T1, T2, T3, T4, T5 of Ciprofol group were higher than those of Propofol group, the differences were statistically significant (P<0.05); HR at T1 and T2 of two groups were higher than those at T0, the differences were statistically significant (P<0.05); HR at T1 and T2 of Ciprofol group were lower than those of Propofol group, the differences were statistically significant (P<0.05); BIS at T1, T2, T3, T4 and T5 of two groups were lower than those at T0, the differences were statistically significant (P<0.05). The induction time, consciousness recovery time and orientation recovery time of Ciprofol group were significantly shorter than those of Propofol group, and the differences were statistically significant (P<0.05 ). The visual analogue scale (VAS) score and incidence of hypotension and hypoxemia in Ciprofol group were lower than those in Propofol group, and the differences were statistically significant (P<0.05). Conclusion: The anesthetic efficacy of 0.4mg/kg Ciprofol was equivalent to

Objective: To assess the therapeutic response of tongue acupuncture in patients with post-stroke dysphagia (PSD). Method: Medical records of PSD patients consecutively admitted to Yangjiang Hospital of Traditional Chinese Medicine between October 2021 and December 2022 were retrospectively analyzed. Eighty patients meeting the inclusion criteria were enrolled. Participants were stratified into a control group (n=40) and a tongue acupuncture group (n=40) based on the specific therapeutic regimen received. The control group was given rehabilitation therapy, while the treatment group was combined with tongue acupuncture. The differences in Water Swallowing Test (WST) grades and Standardized Swallowing Assessment (SSA) scores were compared. The therapeutic outcomes and adverse events were documented following the intervention period. Result: Baseline equivalence in WST grades and SSA scores was observed, demonstrated no significant intergroup differences (P>0.05). Post-therapy, the tongue acupuncture cohort demonstrated greater WST improvement, lower SSA scores, and higher clinical efficacy rates versus controls, differences were statistically significant (P<0.05). No adverse events occurred in either group. Conclusion: Tongue acupuncture combined with rehabilitation achieves superior dysphagia mitigation in PSD compared to rehabilitation alone, with sustained short-term effects observed at 3-month follow-up and an optimal safety trajectory.

Objective: To explore the effect of graded pain management based on numeric rating scales (NRS) in patients with subarachnoid hemorrhage (SAH) after surgery. Method: A total of 83 patients with SAH who underwent interventional embolization in Zhangzhou Hospital from January 2021 to January 2022 were enrolled in the study, the patients were divided into the control group (n=41) and the observation group (n=42) according to the random number table method. The control group was given routine pain management after SAH, and the observation group was given graded pain management based on NRS score. The pain score and self-efficacy score at different times, complications and satisfaction were compared between two groups. Result: There were no significant differences in NRS scores between two groups before and 10 d after intervention (P>0.05); the NRS scores of both groups decreased at 1 d, 3 d, 5 d and 10 d after intervention, the NRS scores in the two group at 1 d, 3 d and 5 d after intervention were significantly lower than those in the control group, the differences were statistically significant (P<0.05). There was no significant difference in the score of general self-efficacy scale (GSES) between two groups before intervention (P>0.05); the GSES scores in the observation group at 1 d, 3 d, 5 d and 10 d

Objective: Preclinical models often fail to capture the different heterogeneity of human malignant tumors, thus lacking clinical predictive ability. Patient derived tumor xenotransplantation (PDX) has become a powerful technique that preserves the molecular heterogeneity of its original samples. Among them, renal subcapsular xenotransplantation technology is a method of establishing a human cancer model in animals, which has received increasing attention in recent years. Based on this technology, medical researchers can study the biological characteristics, histological characteristics, and treatment responses of cancer by transplanting primary tumors obtained from patients into the renal capsule of mice, and then observing long-term reproduction and observation in mice. The article reviewed the progress and application of this technology in clinical and basic medical research in recent years, showcasing its advantages in the field of tumor treatment and research, providing reference for clinical and basic researchers in tumor prevention and treatment research.

Objective: To investigate the expression and significance of mutant P53 protein in the tissues of prophylactic salpingectomy. Method: A total of 929 patients who underwent laparoscopic total or subtotal hysterectomy and bilateral salpingectomy for benign uterine lesions in Xiamen Maternal and Child Health Care Hospital from May 2014 to September 2018 were collected, there were 827 cases of laparoscopic total hysterectomy+bilateral salpingectomy and 102 cases of laparoscopic subtotal hysterectomy+bilateral salpingectomy. The expression of mutant P53 protein in fallopian tube tissue was detected by immunohistochemistry. The factors affecting the expression of mutant P53 protein was analyzed, and followed up the occurrence of serous ovarian cancer after surgery. Result: There were no statistically significant differences in the expression of mutant P53 protein in different fallopian tube tissues among patients with benign uterine disease type, tubal ligation history, cesarean section history and age (P>0.05); however, there was significant difference in the expression of mutant P53 protein in different fallopian tube tissues among patients with the levels of carbohydrate antigen 125 (CA125) (P<0.05). logistic regression analysis showed that CA125 positive was a risk factor for mutant P53 positive protein expression (P<0.05). There was no serous ovarian cancer in the mutant P53 protein positive group after total uterus + bilateral salpingectomy and subtotal uterus + bilateral salpingectomy. Conclusion: The positive rate of mutant P53 protein expression in the tissues of prophylactic salpingectomy was 14.32%, and CA125 positive was a risk factor for mutant P53 positive protein expression, no serous ovarian cancer occurred in these patients after postoperative follow-up.

Objective: To analyze the effect of Snake Antivenins and Agkistrodon Acutus Antivenin in the treatment of trimeresurus stejnegeri bite. Method: A total of 126 patients with trimeresurus stejnegeri bite who admitted to the First Affiliated Hospital of Longyan, Fujian Medical University from January 2019 to December 2022 were included as the study objects, and they were divided into the anti-pit viper group, the anti-five-step snake group and the combined group, with 42 cases in each group. The anti-pit viper group was treated with Snake Antivenins, the anti-five-step snake group was treated with Agkistrodon Acutus Antivenin, and the combined group was treated with Snake Antivenins combined Agkistrodon Acutus Antivenin. The rehabilitation and the blood routine, inflammatory indexes and coagulation function before and after treatment among three groups were compared. Result: The swelling disappearance time of affected limb, pain disappearance time and hospitalization time in the combined group were shorter than those in the anti-pit viper group and the anti-five-step snake group, the swelling disappearance time of affected limb, pain disappearance time and hospitalization time in the anti-five-step snake group were shorter than those in the anti-pit viper group, and the differences were statistically significant (P<0.05). After treatment, the erythrocyte, hemoglobin, white blood cell count, calcitonin, C-reactive protein, serum amyloid A, prothrombin time, thrombin time and fibrinogen in three groups were significantly improved compared with those before treatment, and the differences were statistically significant (P<0.05), the erythrocyte, hemoglobin and fibrinogen in the combined group were higher than those in the anti-pit viper group and the anti-five-step snake group, the white blood cell count, calcitonin, C-reactive protein and serum amyloid A were lower than those in the anti-pit viper group and the anti-five-step snake group, the prothrombin time and thrombin time were shorter than those in the anti-pit viper group and the anti-five-step snake group, and the erythrocyte, hemoglobin and fibrinogen in the anti-five-step snake group were higher than those in the anti-pit viper group, the white blood cell count, calcitonin, C-reactive protein and serum amyloid A in the anti-five-step snake group were lower than those in the anti-pit viper group, and the prothrombin time and thrombin time in the anti-five-step snake group were shorter than those in the anti-pit viper group, the differences were statistically significant (P<0.05). Conclusion: Both Snake Antivenins and Agkistrodon Acutus Antivenin can effectively improve the blood routine, inflammatory response and coagulation function of patients bitten by trimeresurus stejnegeri, the improvement effect of Agkistrodon Acutus Antivenin is better than that of Snake Antivenins, the combination of two can further enhance the clinical efficacy, accelerate the rehabilitation process, and shorten the time of symptom improvement and hospitalization.

Primary Sj.gren's syndrome (PSS) is a chronic inflammatory connective tissue disease characterized by invading exocrine gland, it is more common in female, it can involve multiple organ systems, such as respiratory system, blood system, nervous system, skin, muscle, bone, etc, its prominent symptoms are dry mouth, dry eyes. PSS complicated with depression is more common, the occurrence of depression seriously affects the physical and mental health and quality of life of PSS patients, and brings a very heavy burden to the family and society, it is more and more attention, with the deepening of the research, the pathogenesis of depression in PSS patients is complex and diverse. This paper comprehensively analyzed the pathogenesis of PSS complicated with depression from the aspects of somatic-social-psychological, immune system, neuroendocrine system and cognitive function, in order to improve the treatment methods and quality of life of PSS complicated with depression patients.

Psoriasis is an inflammatory skin disease that occurs under the joint action of multiple factors such as genetics, environment, immunity etc. The interleukin-23 (IL-23) / T helper cell 17 (Th17) pathway is an important research achievement of the pathogenesis of psoriasis in clinical practice. Phosphodiesterase 4 (PDE4) inhibitors had a good blocking effect on cyclic adenosine monophosphate (cAMP) hydrolysis process, and effectively affected IL-23 secretion. This article briefly summarizes the pathogenesis of psoriasis, the mechanism of PDE4 inhibitors in the treatment of psoriasis and the progress of related PDE4 inhibitors drugs in the treatment of psoriasis.

In recent years, chromosome karyotype analysis combined with copy number variation sequencing (CNV-seq) technology is a common means of clinical prevention of birth defects in non-invasive prenatal testing (NIPT) high-risk pregnant women for prenatal diagnosis, detection of fetal serious genetic defects, and clinical intervention after informed consent. Due to its advantages of high sensitivity, specificity, accuracy, and non-invasive, noninvasive prenatal test (NIPT) technology has gradually become the mainstream technology for clinical prenatal screening in recent years. Chromosome karyotype analysis technology has always been considered as the "gold standard" for diagnosing chromosomal aberrations, and is also a first-line method for prenatal diagnosis of chromosomal diseases. The main limitation of chromosome karyotype analysis is that it can not effectively detect the small structural and genetic variations of chromosomes. CNV-seq technology can not only detect conventional chromosome number and structural abnormalities, but also provide more accurate detection information for chromosome fragments from unknown sources. Microdeletion and microduplication of chromosome fragments above 5 Mb can also be detected. However, CNV-seq technology has limitations such as inability to detect balanced translocation and inversion of chromosomes, so the advantages and disadvantages of the two prenatal diagnostic techniques can complement each other. Traditional karyotype analysis combined with CNV-seq technology for prenatal diagnosis will provide more accurate and reliable prenatal diagnosis results for NIPT high-risk pregnant women and reduce birth defects. This article will briefly review the clinical application of chromosome karyotype analysis combined with CNV-seq technology in prenatal diagnosis of NIPT high-risk pregnant women, providing reference for clinical work.

Objective: To analyse the diagnostic value of combining tumor markers carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA199), carbohydrate antigen 242 (CA242) and carbohydrate antigen 724 (CA724) in early gastric cancer. Method: From June 2021 to June 2023, 56 suspected early gastric cancer patients admitted to Clinical Oncology School of Fujian Medical University were selected as the observation group. In addition, 40 patients with benign stomach diseases admitted to in the same period were selected as the control group. The levels of the tumor markers CEA, CA199, CA242 and CA724 were compared between the two groups, and the diagnostic value of the above four tumor markers were tested alone and in combination was analyzed. Result: The levels of CEA, CA199, CA242 and CA724 in observation group were significantly higher than those in control group, the differences were statistically significant (P<0.05). Among the 56 suspected early gastric cancer patients in the observation group, there were 48 positive cases (25 papillary adenocarcinoma cases, 23 tubular adenocarcinoma cases) and 8 negative cases (5 gastric ulcers cases, 3 chronic gastritis cases). The results showed that the sensitivity and accuracy of the combined detection of CEA, CA199, CA242 and CA724 were 93.75% and 92.86%, which were higher than the sensitivity and accuracy of the four separate tests, and the differences were statistically significant (P<0.05). There was no significant difference between the specificity of the combined test and that of the four separate tests (P>0.05). Conclusion: The combined detection of tumor markers CEA, CA199, CA242 and CA724 has high diagnostic value, which is significantly superior to each single detection, and can provide an important theoretical reference for the rapid screening and diagnosis of early gastric cancer.

Objective: To explore the effectiveness of mind mapping health education in patients with colorectal cancer with temporary colostomy. Method: 130 patients with colorectal cancer with temporary enterostomy admitted to Jianyang First Hospital in Nanping City from June 2020 to June 2024 were selected the subjects, and were randomly into the study and the control group, 65 cases in group. The control received conventional rehabilitation nursing care, and the study group used thinking guide health education, and both groups received nursing care for a period of 12 weeks . Before and after the intervention, self-care ability, survival quality, peristoma skin status, awareness and complications were compared between the two groups. Result: After 12 weeks of intervention, erosion, discoloration, and tissue proliferation scores lower in study group compared the control, and difference was statisically sigifiant (P<0.05). The quality of cancer survival scores in the 3 domains of symptomatic domain, functional domain and general health status were elevated in study group compared the control group, the difference was statistcally signifcant (P<0.05). The scores the dimensions of self-care ability the study increased mpared with the trol group, and the diffence was statiscally signcant (P<0.05). Knowledge scores the 3 dimensions of complication recognition, stoma replacement, and precautions were elevated in the study compared with control group, difference was stastically signcant (P<0.05). The complicon rate of the study group was lwer than that of the control, and the difference was stastically signifnt (P<0.05). Conclusion: Mind mapping health education can better improve the quality of cancer survival and peristoma skin status of colorectal cancer patients with temporary colostomy, improve self-care ability and knowledge.

Objective: To observe the effect of Cameron's timely theoretical rehabilitation exercises combined with first-perspective multifaceted evangelism in patients undergoing hip arthroplasty for femoral neck fracture. Method: 94 patients with femoral neck fracture who underwent hip arthroplasty treatment in Putian First Hospital from July 2023 to June 2024 were selected as the study subjects, and were divided into experimental group and control group by coin-flip method, with 47 cases in each group. The control group followed the conventional rehabilitation mode of hip arthroplasty for femoral neck fracture to carry out nursing activities, and the experimental group added Cameron's timely theoretical rehabilitation exercise combined with the first perspective multivariate missionary intervention. The indicators of the two groups after the intervention were compared. Result: The TSK-11 score of the experimental group was lower than that of the control group, the rehabilitation exercise adherence score was higher than that of the control group, the rehabilitation effect indexes were better than that of the control group, and the complication rate was lower than that of the control group, and the difference was statistically significant (P<0.05). Conclusion: The use of Cameron's timely theory of rehabilitation exercise combined with the first perspective multivariate missionary intervention for hip arthroplasty patients with femoral neck fracture can reduce the patients' fear of exercise, improve the patients' adherence to rehabilitation exercise, optimize the patients' rehabilitation effect, and reduce the incidence of complications.

Objective: To investigate the clinical effectiveness of analgesic and sedative comfort nursing combined with seamless care for patients with severe pneumonia and diabetes undergoing mechanical ventilation. Method: A total of 80 patients with severe pneumonia and diabetes undergoing mechanical ventilation admitted to the First Affiliated Hospital of Xiamen University from January to December 2023, were enrolled and randomly assigned using random number table into observation group (n=40) and control group (n=40). The control group received routine care, while the observation group received analgesic and sedative comfort nursing combined with seamless care. Psychological status [Self-rated Anxiety Scale (SAS), Self-rated Depression Scale (SDS) score], treatment compliance, blood glucose levels (fasting and postprandial), duration of mechanical ventilation and hospitalization, as well as the incidence of adverse events were compared between the two groups. Result: After intervention, the observation group showed significantly lower SAS and SDS scores compared to the control group; better treatment compliance and blood glucose control, shorter duration of mechanical ventilation and hospitalization, and a lower incidence of adverse events, the differences were all statistically significant (P<0.05). Conclusion: Analgesic and sedative comfort nursing combined with seamless care can effectively improve the psychological status and treatment compliance of patients with severe pneumonia and diabetes undergoing mechanical ventilation, stabilize blood glucose levels, reduce treatment duration, and lower the risk of adverse events, significantly enhancing overall clinical outcomes.

Objective: To evaluate the efficacy of a precision intercostal nerve block for postoperative analgesia in single-port television-assisted thoracoscopic surgery. Method: 100 patients who underwent single-hole TV-assisted thoracoscopic surgery in the Affiliated Hospital of Qinghai University from January 2022 to January 2023 were selected as the study subjects, and randomly divided into the precise intercostal nerve block (PINB) group and the local infiltration anesthesia (LIA) group, with 50 cases in each group. Pain levels were assessed in the patients' quiet state and deep breathing state in the post-anesthesia recovery room (PACU) (T1), 1 h after leaving the PACU (T2), 4 h (T3), 12 h (T4), and 24 h (T5). The quality of patients' postoperative recovery was assessed at the T5 time point using the 40-item Quality of Recovery Rating Scale (QoR-40). The number of patient controlled intravenous analgesia (PCIA) pump presses and the number of remedial analgesia were recorded for each postoperative period. The time of the first remedial analgesia, patient agitation during awakening, delayed awakening, chills, and the occurrence of adverse reactions such as postoperative nausea and vomiting (PONV), dizziness, skin itching, and hypoxemia during the 24 h postoperative period were recorded. Result: The visual analog scale (VAS) scores at the T2 time point during deep breathing were lower in the PINB group than in the LIA group, and the difference was statistically significant (P<0.05). The VAS at rest and at the T3 time point during deep breathing was lower in the PINB group than in the LIA group, and the difference was statistically significant (P<0.05). The scores of comfort category and pain category in QoR-40 in PINB group were higher than that in LIA group at 24 h after operation, and the difference was statistically significant (P<0.05). The number of PCIA presses and the number of remedial analgesia in PINB group in the time period of T1~T3 was less than that in LIA group, and the difference was statistically significant (P<0.05). The time of the first remedial analgesia in PINB group was shorter than that in LIA group, and the difference was statistically significant (P<0.05). Conclusion: Precision intercostal nerve block provides excellent postoperative analgesia with a high degree of safety in patients undergoing single-port television-assisted thoracoscopic surgery.

The intestinal flora is large in number and variety, which is an important microorganism that determines the level of human health. Maintaining the diversity and abundance of intestinal flora can largely avoid the occurrence of a series of diseases such as intestinal inflammation. This article discusses the effects of phages on human intestinal flora and intestinal inflammation. Based on the relevant studies of many scholars, this article reviews the effects of intestinal phages on intestinal flora and intestinal inflammation, and proposes the use of phages to adjust the function of intestinal flora to treat intestinal inflammation, which provides a new direction for the regulation of intestinal flora and the treatment of intestinal inflammation in the future. We hope to provide some references for readers in the research and work in related fields.

Objective: To investigate the effect of Esketamine combined with Remifentanil and Propofol anaesthesia on pain levels and postoperative awakening in patients undergoing ureteral stent removal. Method: A total of 95 patients who underwent ureteral stent removal in Hai’an Hospital of Traditional Chinese Medicine from June 2022 to June 2023 were selected. Random number table method was used to divide the control group (n=47) and the study group (n=48). The control group was given Remifentanil+Propofol anesthesia, and the study group was given Remifentanil+Propofol+Esketamine anesthesia. Anesthesia effect, pain degree, quality of recovery, cognitive function and incidence of adverse reactions were analyzed and compared between the two groups. Result: The total excellent and good rate of anesthesia in the study group was 95.83%, which was significantly higher than 80.85% in the control group, and the difference was statistically significant (P<0.05). The visual analogue scale (VAS) score of the study group was significantly lower than that of the control group, and the recovery time was earlier than that of the control group, the differences were statistically significant (P<0.05). At 1 h and 3 h after operation, the score of mini-mental state examination (MMSE) in the study group was significantly higher than that in the control group, and the difference was statistically significant (P<0.05). The total incidence of adverse reactions in the study group was 8.33%, which lower than 14.89% in the control group, but there was no significant difference between the two groups (P>0.05). Conclusion: Esketamine combined with Remifentanil and Propofol can significantly reduce the pain of patients undergoing ureteral stent removal, reduce cognitive impairment, shorten postoperative recovery time, and have a high safety.

Objective: To explore the effect of Manchester pain management model (MPMM) combined with enabling education intervention on cesarean section parturient. Method: A total of 100 cases of cesarean section delivered in Women and Children's Hospital Affiliated to Xiamen University from October 2022 to October 2023 were selected, and divided into control group and study group according to different intervention methods, with 50 cases in each group. The control group was treated with routine intervention, and the study group was treated with MPMM combined with enabling education intervention. The degree of postoperative pain, postoperative recovery, negative emotion and exclusive breast-feeding were compared between the two groups. Result: At 8 h, 12 h and 24 h after operation, the numerical rating scale (NRS) scores at rest and exercise of the study group were lower than those of the control group, and the differences were statistically significant (P<0.05). The time of getting out of bed in the study group was earlier than that in the control group, and the difference was statistically significant (P<0.05). After the intervention, the Hamilton anxiety scale (HAMA) scores and Hamilton depression scale (HAMD) scores in the study group were lower than those in the control group, and the differences were statistically significant (P<0.05). The lactation initiation time of the study group was shorter than that of the control group, and the exclusive breast-feeding rate and breast-feeding self-efficacy scale short form (BSES-SF) score of the study group were higher than those of the control group, the differences were statistically significant (P<0.05). Conclusion: MPMM combined with enabling education intervention can reduce pain after uterine labor, promote postoperative recovery, improve maternal negative emotions, and increase the rate of exclusive breast-feeding.

Objective: To analyze the effects of psychological nursing interventions on symptom self-assessment scores and quality of life levels in patients with first-time stroke. Method: A total of 82 patients with first-time stroke admitted to Gaochun People's Hospital in Nanjing from January to November 2024 were selected as the study subjects. They were divided into an observation group and a control group using the envelope method, with 41 cases in each group. The control group received routine nursing intervention, while the observation group received psychological intervention in addition to the routine nursing intervention. The psychological status and quality of life of the two groups were compared. Result: After intervention, anxiety, depression, fear, hostility, paranoia, somatization, obsessive-compulsive symptoms, interpersonal sensitivity, and psychoticism scores decreased in both groups, and the observation group had lower scores, with statistically significant differences (P<0.05). After intervention, both groups showed improvements in energy, mood, personality, thinking, family role, self-care ability, activity ability, and upper limb function scores, and the observation group showed higher scores, with statistically significant differences (P<0.05). Conclusion: Psychological care interventions for patients experiencing their first stroke can effectively improve their psychological condition and quality of life. However, patients often experience severe depression, which requires further targeted measures.

Objective: To compare the therapeutic effects of laparoscopic resection and open abdominal surgery on patients with uterine fibroids (UF). Method: 68 patients with uterine fibroids admitted to the Gaochun People's Hospital in Nanjing from January 2022 to June 2024 were selected and divided into group B (n=34, treated with open abdominal surgery) and group A (n=34, treated with laparoscopic resection) according to different surgical methods. Compare two groups of surgical indicators, Visual Analog Scale (VAS) scores, Generic Quality of Life Inventory (GQOLI-74) scores, and incidence of complications. Result: The surgical indicators in group A were superior to those in group B, and the difference was statistically significant (P<0.05)； The postoperative pain level in group A was lower than that in group B, and the difference was statistically significant (P<0.05)； The postoperative quality of life in group A was higher than that in group B, and the difference was statistically significant (P<0.05)； The incidence of complications in group A was lower than that in group B, and the difference was statistically significant (P<0.05).Conclusion: Compared with open surgery abdominal, laparoscopic resection is more effective in treating patients with uterine fibroids, which can further reduce pain, improve quality of life, and prevent postoperative complications.

Objective: To explore the influence of different postures on patients after laryngeal mask removal in laparoscopic cholecystectomy. Method: According to the random number table method, 80 cases of laparoscopic cholecystectomy in the First Affiliated Hospital of Bengbu Medical University from March 2022 to July 2024 were divided into recumbent group (40 cases with pillows removed) and semi-recumbent group (40 cases with 30° semi-recumbent). The indexes of hemodynamics, pulmonary ventilation and respiratory mechanics of the two groups were compared. Result: There was no significant difference in heart rate (HR) and mean arterial pressure (MAP) at the end of operation, 10 min after laryngeal mask removal, 1 h after laryngeal mask removal and 2 h after laryngeal mask removal between the two groups (P>0.05). Forced vital capacity (FVC), forced expiratory volume in the first second (FEV 1) and FEV 1/FVC in the semi-recumbent group were all higher than those in the recumbent group, and the difference was statistically significant(P<0.05). The maximal expiratory pressure (MEP) and maximal inspiratory pressure (MIP) in the semi-recumbent group were higher than those in the recumbent group at 1 h and 2 h after the laryngeal mask removal, with statistical significance (P<0.05). Conclusion: In laparoscopic cholecystectomy, lying flat without a pillow and 30° semi-lying position will not affect the hemodynamics of patients after laryngeal mask removal, but 30° semi-lying position is more beneficial to the pulmonary ventilation and respiratory mechanics effect of patients.

Objective: To explore the efficacy of mindfulness therapy in schizophrenia rehabilitation period patients. Method: A total of 100 schizophrenic rehabilitation period patients admitted to Qinzhou Psychiatric Hospital from January to October 2022 were selected as the research objects. They were randomly divided into observation group and control group, with 50 cases in each group. Both groups were treated with the same antipsychotic drugs. The control group was administered with routine health education, and the observation group was administered with mindfulness therapy. The medication adherence rate, and anxiety scores, depression scores, quality of life scores before and after the intervention were compared between the two groups. Result: The medication adherence rate of the observation group was 98.00%, which was higher than 80.00% of the control group, the difference was statistically significant (P<0.05). After intervention, the self rating anxiety scale (SAS) score and self rating depression scale (SDS) score of the observation group were (46.63±5.44) points and (47.69±5.56) points respectively, which were lower than (51.61±6.30) points and (53.17±5.72) points of the control group, the differences were statistically significant (P<0.05). The total score of the schizophrenia quality of life scale (SQLS) in the observation group was (20.71±5.21) points, which was significantly lower than (23.86±5.65) points of the control group, and the difference was statistically significant (P<0.05). Conclusion: Implementing mindfulness therapy in schizophrenic rehabilitation period patients can effectively improve the medication adherence, alleviate the anxiety and depression symptoms, improve the cognitive abilities such as attention and memory, enhance the emotional regulation ability, and improve the quality of life after prognosis.

Objective: Observation of the surgical effect of ultrasound-guided percutaneous microwave ablation in the clinical treatment of benign thyroid nodules. Method: 284 patients with benign thyroid nodules from Shenzhen Hengsheng Hospital from July 2021 to July 2024 were selected as the study subjects. The grouping method was envelope randomization. The control group (142 cases) underwent conventional endoscopic unilateral thyroidectomy, while the study group (142 cases) underwent ultrasound-guided percutaneous microwave ablation. The clinical efficacy of the two groups was compared. Result: The appearance satisfaction, postoperative free triiodothyronine (FT3) levels, free tetraiodothyronine (FT4) levels and total thyroxine (TT4) levels of the study group were all higher than those of the control group, with significant difference (P<0.05). The postoperative thyroid stimulating hormone (TSH) levels, adrenocorticotropic hormone (ACTH) levels, aldosterone (ALD) levels, cortisol (Cor) levels, total white blood cell count (WBC) levels, tumor necrosis factor-α (TNF-α) levels, C-reactive protein (CRP) levels, and pain index (VAS) scores of the study group were all lower than those of the control group, with significant difference (P<0.05). The improvement of perioperative indicators in the research group was better than that in the control group, with significant difference (P<0.05). Postoperative complications are comparable between the two groups with no signigicant difference (P>0.05). Conclusion: Ultrasound guided percutaneous microwave ablation for the treatment of benign thyroid nodules can promote postoperative appearance satisfaction, reduce thyroid function damage, alleviate postoperative traumatic stress and inflammatory reactions, improve rehabilitation efficiency, and improve prognosis.

Dexamethasone is a synthetic long-acting glucocorticoid, a fluorinated derivative of prednisolone, with anti-inflammatory, antitoxic, anti-immune and antishock effects, excitation of the central nervous system, affecting the hematopoietic system, promoting the secretion of gastric acid, inhibiting the pineal gland's secretion of melatonin, and decreasing iodine ion uptake by the thyroid gland, which has been widely used in clinical practice. For unexplained fever and osteoarthritic pain, it has the effect of temporarily reducing fever and alleviating pain, which is favored by clinicians, especially in the primary health care institutions is a large number of applications, and even reached the point of abuse. The article found that dexamethasone would have adverse reactions such as ergotism, hypokalemic paralysis, anaphylactic shock, mental abnormality, perineal itching, urinary incontinence, acanthosis nigricans, epilepsy, gastric perforation, and femoral head necrosis in clinical application, which would cause unnecessary pain to patients. These adverse reactions occurring in the clinical application of dexamethasone in recent years are now analyzed and summarized to provide reference for future clinical application.

Objective: To investigate the effects of ultrasound-guided dual nerve block technology on analgesia, motor function preservation, and postoperative recovery in ankle surgery patients. Method: A total of 80 patients undergoing ankle surgery at Xinhui District Hospital of Traditional Chinese Medicine in Jiangmen City, Guangdong Province from April to November 2024 were selected as subjects. The study was divided into experimental and control groups using equalized sequential matching. Equalized sequential matching was applied, with 40 cases in the control group receiving combined spinal-epidural anesthesia and 40 cases in the experimental group receiving ultrasound-guided popliteal popliteal nerve block combined with obturator block. The effectiveness of the control anesthesia protocol during surgical treatment was evaluated. Result: Analysis of heart rate, systolic blood pressure, diastolic blood pressure, and oxygen saturation levels showed no significant differences across time periods. Systolic and diastolic blood pressure remained unchanged before anesthesia initiation and at the end of surgery. However, the experimental group exhibited higher blood pressure at the start of surgery and 30 minutes post-surgery compared to the control group, with statistically significant difference (P<0.05). No significant difference was observed at 1 h postoperative, but statistically significant differences (P<0.05) were found at 12 h and 24 h postoperative, indicating better analgesia outcomes. The experimental group also demonstrated superior nerve block efficacy compared to the control group (P<0.05). Conclusion: During ankle surgery, implementing ultrasound-guided popliteal popliteal nerve block combined with obturator block ensures reliable anesthesia blockade, maintains stable circulatory status, and delivers high-quality analgesia.

Spinal cord injury disrupts the structural and functional connections between the central nervous system and the spinal cord, resulting in severe motor, sensory, and autonomic nerve dysfunctions, which seriously affect the daily living ability of patients. Transcranial magnetic stimulation is a safe, effective and non-invasive neural regulation technology, which is beneficial to the motor function recovery after spinal cord injury. This article mainly reviews transcranial magnetic stimulation in terms of assessment and treatment of motor function after spinal cord injury.

Objective: To observe and analyze the effect of Sertraline combined with Tandospirone in the treatment of adolescent depression patients with non-suicide self-injury behavior (NSSI). Method: A total of 72 adolescent depression patients with NSSI who treated in Fujian Province Fuzhou Neuropsychiatric Hospital from October 2022 to January 2023 were selected as the research objects, the patients were divided into the control group and the observation group by random number table method according to the order of hospitalization, with 36 cases in each group. Both groups were treated with Sertraline Hydrochloride Tablets, and the observation group was treated with Tandospirone Citrate Capsules. The clinical efficacy, negative emotions [Hamilton depression scale-17 item (HAMD-17) score, Hamilton anxiety scale (HAMA) score], impulsive behavior [impulse scale (BIS-11)], NSSI behavior and adverse reactions were compared between two groups. Result: The total effective rate in the observation group was higher than that in the control group, the difference was statistically significant (P<0.05). There were no statistically significant differences in HAMD-17 score and HAMA score between two groups before treatment (P>0.05); after treatment, the HAMD-17 score and HAMA score of two groups were lower than those before treatment, which in the observation group were lower than those in the control group, the differences were statistically significant (P<0.05). Before treatment, there were no significant difference in the attention, exercise, lack of planning and total scores between two groups (P>0.05); after treatment, the attention, exercise, lack of planning and total scores of two groups were lowere than those before treatment, and which in the observation group were lower than the control group, the differences were statistically significant (P<0.05). Before treatment, there were no significant difference in the frequency of occurrence of NSSI behavior between two groups (P>0.05); after 2 weeks and 4 weeks of treatment, the frequency of occurrence of NSSI behavior in two groups were less than those before treatment, and which in the observation group were less than those in the control group, the differences were statistically significant (P<0.05). There was no significant difference in the incidence of adverse reactions between two groups (P>0.05). Conclusion: Sertraline combined with Tandospirone in the treatment of adolescent depression patients with NSSI can significantly improve anxiety and depression, improve impulsive behavior and NISSI behavior, and will not increase adverse reactions.

Breast cancer is the cancer with the highest incidence rate at present, the recurrence and metastasis of breast cancer is one of the unsolved clinical problems. With the development of traditional Chinese medicine, traditional Chinese medicine has played an irreplaceable role in the treatment of recurrent and metastatic breast cancer, and has achieved good clinical efficacy. Through literature research, this paper discussed the clinical research progress of traditional Chinese medicine in the recognition, pathogenesis and prevention of recurrent metastatic breast cancer, and systematically evaluated the advantages of traditional Chinese medicine and its shortcomings that still need to be improved.

Objective: To explore the application effect of new surgical film and optical cable storage tray for operation in operating room management. Method: Random number table method from January to December 2021 to the Second Affiliated Hospital of Nanhua University after treated 280 patients with urinary calculi surgery was divided into A, B, C, D four groups, each group of 70 cases. Group A was treated with a new surgical film, group B with surgical optical cable storage tray, group C with conventional intervention, group D with a new surgical film combined with surgical optical cable storage tray. The infection status of each group, the satisfaction of patients and medical staff were compared, and the cost was calculated. Result: There was significant difference in the incidence of infection among four groups (P<0.05); the incidence of infection in group D was lower than those in group A, group B and group C, the differences were statistically significant (P<0.05). There was significant difference in the nursing satisfaction of patients among four groups (P<0.05); the nursing satisfaction of patients in group D was higher than those of group A, group B and group C, the differences were statistically significant (P<0.05). There was significant difference in the satisfaction of surgical medical staff among four groups (P<0.05); the satisfaction of surgical medical staff in group D was higher than that in group C, the differences was statistically significant (P<0.05). There was significant difference in the cost accounting among four groups (P<0.001); the economic cost in group D was lower than that in group C, but which was higher than those in group A and group B, the differences were statistically significant (P<0.05). Conclusion: The new surgical film and optical cable storage tray in the operation process of patients with urinary calculi surgery can not only reduce the infection in the operating room, but also improve the satisfaction of patients and medical staff, and reduce the cost of medical consumables.

Objective: To explore the application effect of prenatal perineal massage combined with psychological counseling in primiparas. Method: A total of 200 primiparas admitted to the Zhongshan People's Hospital from June 2023 to June 2024 were selected as the research subjects and divided into two groups by envelope method, with 100 cases in each group. Both groups received conventional care. The control group was combined with psychological counseling, while the observation group was combined with prenatal perineal massage on the basis of the control group. Both groups completed 3 d care. The psychological resilience, stress response, role transition scores and delivery outcomes of the two groups were compared. Result: The observation group had a higher score on the Connor-Davidson Resilience Scale (CD-RISC) after intervention than the control group, with a statistically significant difference (P<0.05). Both groups of primiparous women had varying degrees of stress response, but the degree of stress response in the observation group was milder than that in the control group. The stress response indicators in the observation group were lower than those in the control group. The role transition score and normal delivery rate in the observation group were higher than those in the control group after intervention, and the rate of instrumental delivery was slightly lower than that in the control group, with a statistically significant difference (P<0.05). Conclusion: Prenatal perineal massage combined with psychological counseling for primiparas can help improve their psychological resilience, reduce the stress response during delivery, increase the role transition score, reduce the incidence of instrument delivery, and increase the rate of normal delivery.

Objective: To observe the effect of single-port thoracoscopic radical resection of lung cancer on perioperative indicators and survival in patients with early non-small cell lung cancer (NSCLC). Method: The clinical data of 40 patients with NSCLC who underwent single-port thoracoscopic radical resection of lung cancer in Qiandongnan People's Hospital from July 2018 to November 2021 were retrospectively collected and included in group A. In addition, the clinical data of 40 patients with NSCLC who underwent three-port thoracoscopic radical resection of lung cancer in our hospital during the same period were collected and included in group B. The surgical indexes, postoperative pain [visual analogue scale (VAS)], stress indexes [cortisol (Cor), norepinephrine (NE), angiotensin Ⅱ (Ang Ⅱ)], postoperative complications and survival were compared between two groups. Result: The operation time of group A was longer than that of group B, the chest tube indwelling time and hospitalization time were shorter than those of group B, and the differences were statistically significant (P<0.05); the VAS scores of group A at 12 h, 24 h and 48 h after operation were lower than those of group B, and the differences were statistically significant (P<0.05); at 1 d after operation, Cor, NE and Ang Ⅱ in two groups increased, but those in group A were lower than those in group B, and the differences were statistically significant (P<0.05); the incidence of postoperative complications in group B was higher than that in group A, the difference was statistically significant (P<0.05); there were no significant differences in the 1-year and 3-year survival rates between two groups (P>0.05). Conclusion: Single-port thoracoscopic radical resection of lung cancer in the treatment of early NSCLC patients has less trauma, which can reduce postoperative stress response, relieve postoperative pain, facilitate postoperative recovery, and shorten hospitalization time, and the survival rate is high.

Objective: To investigate the correlation among hepatic transient elastography index, spleen thickness, portal vein inner diameter, fibrosis 4 index (FIB4), aspartate aminotransferase to platelet ratio index (APRI) and the degree of esophageal varices in liver cirrhosis. Method: From December 2020 to February 2022, 40 patients with severe esophageal varices in liver cirrhosis admitted to the Second People's Hospital of Tianjin were selected as group A, 40 patients with moderate esophageal varices in liver cirrhosis were selected as group B, 40 patients with mild esophageal varices in liver cirrhosis were selected as group C, and 40 patients without esophageal varices in liver cirrhosis were selected as group D. Hepatic transient elastography, alanine aminotransferase (ALT), aspartate aminotransferase (AST), platelet (PLT) detection were performed in all the four groups, and FIB4 and APRI were calculated. The hepatic transient elastometry indexes, spleen thickness, portal vein inner diameter, FIB4 and APRI of four groups were compared. Spearman rank correlation was used to analyze the relationship among the above detection indexes and the severity of esophageal varices in liver cirrhosis. Result: The liver stiffness value, spleen stiffness value, spleen thickness and portal vein inner diameter, FIB4 and APRI in group A, group B and group C were significantly higher than those in group D, the liver stiffness value, spleen stiffness value, spleen thickness and portal vein inner diameter, FIB4 and APRI in group A and group B were significantly higher than those in group C, the liver stiffness value, spleen stiffness value, spleen thickness and portal vein inner diameter, FIB4 and APRI of group A were higher than those of group B, and the differences were statistically significant (P<0.05). Spearman rank correlation analysis showed that liver stiffness value, spleen stiffness value, spleen thickness, portal vein inner diameter, FIB4, APRI were positively correlated with the degree of esophageal varices in liver cirrhosis (P<0.05). Conclusion: The expression of hepatic transient elastography index, spleen thickness, portal vein inner diameter and FIB4, APRI of patients with liver cirrhosis esophageal varices are significantly abnormal, and the above indicators are closely related to the degree of esophageal varices, so their detection value in the patients with liver cirrhosis esophageal varices is high.

Objective: To compare the differences between percutaneous microballoon compression and radiofrequency thermocoagulation in the treatment of primary trigeminal neuralgia. Method: A retrospective review of clinical data from 86 patients with primary trigeminal neuralgia admitted to Yancheng NO.1 People's Hospital between August 2019 and August 2023 was conducted, and the patients were divided into two groups based on treatment modality: the balloon group and the radiofrequency group, with 43 patients in each group. The balloon group received percutaneous microballoon compression therapy, while the radiofrequency group received radiofrequency thermal coagulation therapy. The clinical efficacy, pain levels, recurrence rates, and incidence of complications were compared between the two groups. Result: After 3 months postoperatively, there was no statistically significant difference in the overall clinical efficacy rate between the two groups (P>0.05). Compared with 1 month postoperatively, the visual analog scale scores were higher in both groups at 3 months postoperatively, with a statistically significant difference (P<0.05). The recurrence rate was higher in the radiofrequency group than in the balloon group, but the difference was not statistically significant (P>0.05). The incidence of facial numbness and masseter muscle weakness was lower in the radiofrequency group than in the balloon group, while the incidence of corneal discomfort was higher in the radiofrequency group than in the balloon group, and both differences were statistically significant (P<0.05). There was no statistically significant difference in the incidence of oral herpes between the two groups (P>0.05). Conclusion: Microballoon compression and radiofrequency thermocoagulation are similar in their effectiveness in treating patients with primary trigeminal neuralgia. Both procedures result in similar pain relief and recurrence rates one year after surgery. However, percutaneous microballoon compression has a lower incidence of complications such as facial numbness and masseter muscle weakness, but a higher incidence of corneal discomfort.

Lumbar disc herniation (LDH) is a common condition in clinical practice, particularly in orthopedics, often causing lower back and leg pain and affecting patients' quality of life. Traditional Chinese medicine acupuncture has been shown to be effective in treating LDH patients, offering advantages such as simplicity, convenience, affordability and reliability, and plays an irreplaceable role in treatment. The article reviews relevant literature on the mechanisms of action of acupuncture in the treatment of LDH over the past five years and summarizes the findings. The results indicate that acupuncture treatment for LDH is closely associated with multiple mechanisms of action, including reducing inflammation levels, improving bone metabolism, improving serum differential metabolite levels, regulating neurotransmitters, enhancing nerve conduction velocity, and improving blood rheology.

Objective: To explore the shunt value of gene methylation testing combined with high-risk HPV testing for cervical cancer screening. Method: The clinical data of 80 women who underwent opportunistic screening at the Cervical Disease Diagnosis and Treatment Center of Quanzhou First Hospital from October 2020 to October 2022 was collected, all of whom underwent gene methylation testing, high-risk HPV testing, and cervical pathological biopsy under colposcopy. Using the results of cervical pathological biopsy as the gold standard, and the consistency of gene methylation testing, high-risk HPV testing alone and in combination with the gold standard, and the diagnostic efficacy of cervical cancer, and the screening diversion of cervical cancer were compared. Result: Among the 80 cases of cervical cancer screening, a total 23 cases were positive in cervical pathological biopsy results, accounting for 28.75% (23/80), and 57 cases were negative, accounting for 71.25% (57/80). Among the 23 positive cases, cervical squamous cell carcinoma (SCC) accounted for 43.48% (10/23), low-grade squamous intraepithelial lesions (LSIL) accounted for 39.13% (9/23), and high grade squamous intraepithelial lesions (HSIL) accounted for 17.39% (4/23). There were 16 cases diagnosed with cervical cancer by gene methylation testing, and 15 cases diagnosed with cervical cancer by high-risk HPV testing, which showed poor consistency with the gold standard (Kappa=0.372, 0.357); there were 19 cases diagnosed with cervical cancer by combining the two tests, which showed good consistency with the gold standard (Kappa=0.784). The specificity, accuracy, and negative predictive value of gene methylation testing combined with high-risk HPV testing for screening cervical cancer were significantly higher than those of gene methylation testing and high-risk HPV testing alone, the differences were statistically significant (P<0.05). There were no statistically significant differences in the sensitivity and positive predictive value of the combined test compared to the two tests alone (P>0.05). The positive testing rate of SCC in combination testing was higher than that in individual testing, and the referral rate of colposcopy was lower than that in individual testing, the differences were statistically significant (P<0.05). There were no statistically significant differences in the positive testing rates of HSIL and LSIL detected by gene methylation testing, high-risk HPV testing and combination testing (P>0.05). Conclusion: The combination of gene methylation testing and high-risk HPV testing has high value in the diagnosis of cervical cancer. It can improve the positive diagnosis rate of cervical cancer and reduce the referral rate of colposcopy through shunt testing in cytological abnormalities.

Objective: To observe the effect of treatment of refractory diabetic macular edema (DME) using intravitreal dexamethasone extended-release injection versus ranibizumab. Method: 68 patients with refractory DME admitted to Sanmenxia Hospital of Yellow River from June 2022 to June 2024 were selected as the study subjects, and were divided into the experimental group (dexamethasone extended-release agent combined with intravitreal injection of ranibizumab, 36 cases) and the control group (intravitreal injection of ranibizumab, 32 cases) according to the different modes of treatment. Retinal thickness (CMT), intraocular pressure (IOP), best corrected visual acuity (BCVA), number of injections of anti-vascular endothelial growth factor (VEGF) medication, recurrence of edema, and complication rate were compared between the two groups. Result: At 6 months of treatment, the difference in IOP between the two groups was not statistically significant (P>0.05). Compared with the control group, the experimental group had lower BCVA (logMAR) and CMT, fewer recurrences of edema, longer duration of edema recurrence, fewer injections of anti-VEGF drugs and lower overall complication rate, and the differences were all statistically significant (P<0.05). Conclusion: For refractory DME, the application of intravitreal dexamethasone extended-release injections with ranibizumab for treatment improves the patient's visual acuity and condition, reduces recurrence of edema, reduces the frequency of anti-VEGF drug injections, and enhances safety.

Objective: To analyze the effects of a new perineal massage technique combined with a responsibility-based one-to-one nursing model on labor progression and delivery outcomes in primiparous women. Method: A total of 200 primiparous women who delivered at the Zunyi Maternal and Child Health Hospital from April to November 2024 were selected as study subjects. They were divided into a control group (96 cases) and an observation group (104 cases) based on differences in nursing methods during delivery. The control group received one-to-one nursing care under the responsibility system, while the observation group received the new perineal massage technique in addition to one-to-one nursing care under the responsibility system. The two groups were compared in terms of labor progression and delivery outcomes. Result: There was no statistically significant difference in the specific duration of the first and third stages of labor between the two groups (P>0.05). The duration of the second stage of labor was significantly shorter in the observation group compared to the control group (P<0.05) . The incidence of perineal side incisions, tears, and the severity of perineal edema were lower in the observation group than in the control group, with statistically significant differences (P<0.05). There were no statistically significant differences between the two groups in terms of cesarean section rates and postpartum complications (P>0.05). Conclusion: The new perineal massage technique combined with a responsibility-based one-to-one nursing model can accelerate the second stage of labor in primiparous women, protect perineal integrity, and result in better delivery outcomes.